Melo Silvia V, Agnes Grasiela, Vitolo Márcia R, Mattevi Vanessa S, Campagnolo Paula D B, Almeida Silvana
Laboratório de Biologia Molecular, Universidade Federal de Ciências da Saúde de Porto Alegre, Porto Alegre, RS, Brazil.
Programa de Pós-Graduação em Ciências da Saúde, Universidade Federal de Ciências da Saúde de Porto Alegre, Porto Alegre, RS, Brazil.
Genet Mol Biol. 2017 Apr-Jun;40(2):415-420. doi: 10.1590/1678-4685-GMB-2016-0205. Epub 2017 May 11.
Taste perception plays a key role in determining individual food preferences and dietary habits and may influence nutritional status. This study aimed to investigate the association of TAS1R2 (Ile191Val - rs35874116) and TAS1R3 (-1266 C/T - rs35744813) variants with food intake and nutritional status in children followed from birth until 7.7 years old. The nutritional status and food intake data of 312 children were collected at three developmental stages (1, 3.9 and 7.7 years old). DNA was extracted from blood samples and the polymorphisms were analyzed by real-time polymerase chain reactions (qPCR) using hydrolysis probes as the detection method. Food intake and nutritional status were compared among individuals with different single nucleotide polymorphism (SNP) genotypes. At 3.9 years old, children homozygous (Val/Val) for the TAS1R2 Ile191Val polymorphism ingested less sugar and sugar-dense foods than children who were *Ile carriers. This finding demonstrated that a genetic variant of the T1R2 taste receptor is associated with the intake of different amounts of high sugar-content foods in childhood. This association may provide new perspectives for studying dietary patterns and nutritional status in childhood.
味觉感知在决定个体食物偏好和饮食习惯方面起着关键作用,并可能影响营养状况。本研究旨在调查从出生到7.7岁儿童的TAS1R2(Ile191Val - rs35874116)和TAS1R3(-1266 C/T - rs35744813)基因变异与食物摄入量和营养状况之间的关联。在三个发育阶段(1岁、3.9岁和7.7岁)收集了312名儿童的营养状况和食物摄入量数据。从血样中提取DNA,并使用水解探针作为检测方法,通过实时聚合酶链反应(qPCR)分析多态性。比较了具有不同单核苷酸多态性(SNP)基因型个体的食物摄入量和营养状况。在3.9岁时,TAS1R2 Ile191Val多态性纯合子(Val/Val)的儿童比携带Ile的儿童摄入的糖和高糖食物更少。这一发现表明,T1R2味觉受体的基因变异与儿童期不同量的高糖食物摄入有关。这种关联可能为研究儿童期饮食模式和营养状况提供新的视角。