Lin Qing-Shuang, Huang Yue-Lin, Fan Xiao-Xing, Zheng Xiao-Lan, Chen Xiao-Lan, Zhan Xin-Qi, Zheng Hong
Department of Chemistry, College of Chemistry and Chemical Engineering, and the MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, Xiamen University, Xiamen 361005, China; Basic Medicine Department, Medical College of Xiamen University, Xiamen 361102, China.
Department of Chemistry, College of Chemistry and Chemical Engineering, and the MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, Xiamen University, Xiamen 361005, China.
Talanta. 2017 Aug 1;170:496-501. doi: 10.1016/j.talanta.2017.04.024. Epub 2017 Apr 12.
An interesting ratiometric fluorescent probe with unique optical performance was reported in this work. By modifying on the bridge-head of heptamethine cyanine chromophore with an N-phenyl-N'-ethylene amine thiourea substituent as a chemodosimetric recognition unit, the probe exhibited ratiometric fluorescent response towards hypochlorous acid (HClO). Upon addition of HClO, the absorbance spectra showed a great red shift as large as 150nm from 650nm to 800nm. Employing the isosbestic absorption point at 730nm as an excitation wavelength, a ratiometric fluorescent sensing mode with two long emission wavelengths at 760nm and 820nm was acquired, and thus the probe displayed significant behavior with both the excitation wavelength and the dual-emission wavelengths located at NIR (650-900nm) region exclusively. Also, the probe showed excellent performance in high sensitivity and good selectivity towards HClO over other reactive oxygen species and a wide variety of coexist species in biological pH condition and had been successfully used to detect hypochlorous acid in serum samples and tap water samples.
本研究报道了一种具有独特光学性能的有趣的比率荧光探针。通过在七甲川花菁发色团的桥头修饰一个N-苯基-N'-乙烯胺硫脲取代基作为化学计量识别单元，该探针对次氯酸（HClO）表现出比率荧光响应。加入HClO后，吸收光谱显示出从650nm到800nm高达150nm的巨大红移。以730nm处的等吸收点作为激发波长，获得了在760nm和820nm处有两个长发射波长的比率荧光传感模式，因此该探针在激发波长和双发射波长均仅位于近红外（650-900nm）区域时表现出显著特性。此外，该探针在生物pH条件下对HClO具有高灵敏度和良好的选择性，对其他活性氧和多种共存物质表现优异，已成功用于检测血清样品和自来水样品中的次氯酸。
