Hasani Z, Aghdaei H Asadzadeh, Balaii H, Azimirad M, Mirsamadi E S, Mirjalali H, Zali M R
Foodborne and Waterborne Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences,Tehran,Iran.
Basic and Molecular Epidemiology of Gastrointestinal Disorders Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences,Tehran,Iran.
Epidemiol Infect. 2017 Jul;145(10):2095-2099. doi: 10.1017/S0950268817000954. Epub 2017 May 15.
Microsporida are known as opportunistic unicellular organisms and have recently been reclassified as fungi that have been frequently reported from patients with congenital and acquired immunity failure disorders, worldwide. However, use of immunosuppressive medications in inflammatory bowel disease (IBD) patients significantly decreases overall immunity, and increases their susceptibility to opportunistic infections. Totally, 71 stool samples were collected from IBD patients consisted of 69 ulcerative colitis (UC) patients and two Crohn's disease (CD) patients. All patients had taken immunosuppressive and/or immunomodulator drugs for at least 3 weeks. DNA was extracted from all stool samples and Nested PCR was performed using genus-specific primers based on small subunit ribosomal RNA (SSU rRNA) gene. Fisher's Exact Test was applied to evaluate statistical association between microsporidia infection and sex, age and types of IBD. Mean of age ± s.d., women and men percentage of the attended patients were 36·17 ± 11·93, 60·6%, and 39·4%, respectively. A 440-bp fragment of SSU rRNA gene attributed to Enterocytozoon bieneusi was amplified from 12·7% of IBD patients. No Encephalitozoon DNA was detected in the samples. No microsporidia-positive sample was found in CD patients. Fisher's Exact Test showed that there was no statistically significant correlation between intestinal microsporidiosis and age, sex, and IBD types with P values: 0·389, 1·00, and 1·00, respectively. This study has shown IBD patients undergoing immunosuppressive/immunomodulators medications, which may be susceptible to intestinal microsporida infection. E. bieneusi is the commonest intestinal microsporidan reported from IBD patients.
微孢子虫是已知的机会性单细胞生物，最近被重新归类为真菌，在全球范围内，先天性和获得性免疫功能衰竭疾病患者中经常报告有微孢子虫。然而，炎症性肠病（IBD）患者使用免疫抑制药物会显著降低整体免疫力，并增加他们对机会性感染的易感性。总共从IBD患者中收集了71份粪便样本，其中包括69例溃疡性结肠炎（UC）患者和2例克罗恩病（CD）患者。所有患者均服用免疫抑制和/或免疫调节剂药物至少3周。从所有粪便样本中提取DNA，并使用基于小亚基核糖体RNA（SSU rRNA）基因的属特异性引物进行巢式PCR。应用Fisher精确检验来评估微孢子虫感染与性别、年龄和IBD类型之间的统计学关联。就诊患者的年龄平均值±标准差、女性和男性百分比分别为36.17±11.93、60.6%和39.4%。从12.7%的IBD患者中扩增出了归因于比氏肠微孢子虫的440bp SSU rRNA基因片段。样本中未检测到脑孢子虫DNA。在CD患者中未发现微孢子虫阳性样本。Fisher精确检验表明，肠道微孢子虫病与年龄、性别和IBD类型之间无统计学显著相关性，P值分别为0.389、1.00和1.00。这项研究表明，接受免疫抑制/免疫调节剂药物治疗的IBD患者可能易患肠道微孢子虫感染。比氏肠微孢子虫是IBD患者中报告的最常见的肠道微孢子虫。
