Shi Li, Wang Mengyao, Zhang Yichao, Shen Guangmao, Di Haishan, Wang Yue, He Lin
Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing 400716, China.
Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing 400716, China.
Comp Biochem Physiol C Toxicol Pharmacol. 2017 Aug;198:28-36. doi: 10.1016/j.cbpc.2017.05.002. Epub 2017 May 11.
Although overexpression of genes encoding detoxification enzymes is a well-known mechanism of pesticide resistance of mites, the regulators involved in this process are still illiterate. Previous studies in our laboratory demonstrated that the overexpression of six P450 genes contributes to fenpropathrin resistance in T. cinnabarinus. In this study, six transcription factor genes that likely regulate the expression of P450 genes were identified and characterized. Quantitative PCR (qPCR) analysis showed that three transcription factor genes were highly expressed in a fenpropathrin-resistant (FeR) strain of T. cinnabarinus. The cap 'n' collar isoform C (CncC) and muscle aponeurosis fibromatosis (Maf) family transcription factors were identified as the key regulator of P450 genes by RNA interference (RNAi). Furthermore, research on the promoters of these P450 genes using reporter assays identified that CncC and Maf influence the susceptibility of T. cinnabarinus to fenpropathrin through regulating the expression of P450 genes. This study increases our understanding of the molecular mechanisms underlying the regulation of P450 genes involved in detoxification of acaricides in T. cinnabarinus.
尽管编码解毒酶的基因过表达是螨类对杀虫剂产生抗性的一种众所周知的机制，但参与这一过程的调控因子仍不清楚。我们实验室之前的研究表明，六个P450基因的过表达导致朱砂叶螨对甲氰菊酯产生抗性。在本研究中，鉴定并表征了六个可能调控P450基因表达的转录因子基因。定量PCR（qPCR）分析表明，三个转录因子基因在朱砂叶螨的甲氰菊酯抗性（FeR）品系中高表达。通过RNA干扰（RNAi）确定，帽领异构体C（CncC）和肌腱膜纤维瘤病（Maf）家族转录因子是P450基因的关键调控因子。此外，使用报告基因分析对这些P450基因的启动子进行研究发现，CncC和Maf通过调控P450基因的表达影响朱砂叶螨对甲氰菊酯的敏感性。本研究增进了我们对朱砂叶螨中参与杀螨剂解毒的P450基因调控的分子机制的理解。
