Fu Qianyan, Zeng Tian, Xu Yijuan
Guangdong Laboratory for Lingnan Modern Agriculture, Department of Entomology, South China Agricultural University, Guangzhou 510642, China.
Insects. 2022 Aug 30;13(9):785. doi: 10.3390/insects13090785.
The cap 'n' collar isoform C () transcription factor is thought to be a regulator associated with antioxidant and detoxification genes that can enhance pest resistance by regulating the expression of detoxification enzyme genes. However, this transcription factor has not been well studied in the important agricultural pest . In this study, the cDNA sequence of in was cloned, and the complete ORF sequence was obtained; it had a sequence length of 3378 bp, encoding a total of 1125 amino acids. Phylogenetic tree analysis showed that belonged to the CNC family and that its amino acid sequence showed the closest relationship with . The conserved structural region of was analyzed and was found to include a conserved bZIP superfamily structural domain. Spatiotemporal expression analysis revealed that was most highly expressed in the adult Malpighian tubules, followed by the antennae, foregut, and midgut, and then the brain, hemolymph, hindgut, and fat body. was expressed at every developmental stage, and the highest expression was found in mature males. This study provides a theoretical basis for an in-depth investigation of the function of in regulating pesticide resistance in .
帽领异构体C（）转录因子被认为是一种与抗氧化和解毒基因相关的调节因子，它可以通过调节解毒酶基因的表达来增强害虫抗性。然而，这种转录因子在重要农业害虫中尚未得到充分研究。在本研究中，克隆了该害虫中的cDNA序列，并获得了完整的开放阅读框序列；其序列长度为3378 bp，共编码1125个氨基酸。系统发育树分析表明，该转录因子属于CNC家族，其氨基酸序列与关系最为密切。对该转录因子的保守结构区域进行分析，发现其包含一个保守的bZIP超家族结构域。时空表达分析显示，该转录因子在成年马氏管中表达最高，其次是触角、前肠和中肠，然后是脑、血淋巴、后肠和脂肪体。在每个发育阶段均有表达，在成熟雄性中表达最高。本研究为深入研究该转录因子在调节该害虫抗药性中的功能提供了理论依据。
