Tang Guang-Hui, Xiong Ying, Liu Yi, Song Zhong-Hao, Yang Yang, Shen Guang-Mao, Wang Jin-Jun, Jiang Hong-Bo
Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing, China.
State Cultivation Base of Crop Stress Biology for Southern Mountainous Land, Southwest University, Chongqing, China.
Front Physiol. 2019 Aug 20;10:1068. doi: 10.3389/fphys.2019.01068. eCollection 2019.
Pesticide resistance is a serious problem that poses a major challenge to pest control. One of the most potent resistance mechanisms is the overexpression of genes coding for detoxification enzymes. The expression of detoxification genes is regulated by a series of transcription factors. Previous studies have revealed that the increased expression of detoxification genes contributes to the insecticide tolerance of Our objective was thus to identify the transcription factors involved in this process. Temporal expression profiles showed that the transcription factor and detoxification genes were expressed highly in the fat body. Further analysis showed that the expression of , , and was induced by abamectin. Disruption of the transcription factor through RNA interference decreased the transcript levels of and and increased the susceptibility to abamectin significantly. Direct silencing of the expression of also increased susceptibility to abamectin, while did not. In conclusion, these results suggest that the expression of and was regulated by the transcription factor , and the up-regulation of via decreased the susceptibility of to abamectin.
抗药性是一个严重问题，对害虫防治构成重大挑战。最有效的抗性机制之一是编码解毒酶的基因过度表达。解毒基因的表达受一系列转录因子调控。先前的研究表明，解毒基因表达增加有助于昆虫对杀虫剂产生耐受性。因此，我们的目标是鉴定参与这一过程的转录因子。时间表达谱显示，转录因子和解毒基因在脂肪体中高表达。进一步分析表明，阿维菌素可诱导[具体转录因子名称1]、[具体转录因子名称2]和[具体转录因子名称3]的表达。通过RNA干扰破坏[具体转录因子名称1]转录因子会降低[具体解毒基因名称1]和[具体解毒基因名称2]的转录水平，并显著增加对阿维菌素的敏感性。直接沉默[具体解毒基因名称1]的表达也会增加对阿维菌素的敏感性，而[具体解毒基因名称2]则不会。总之，这些结果表明，[具体解毒基因名称1]和[具体解毒基因名称2]的表达受转录因子[具体转录因子名称1]调控，通过[具体转录因子名称1]上调[具体解毒基因名称1]会降低[具体昆虫名称]对阿维菌素的敏感性。
