供体血浆中巨细胞病毒抗体的酶联免疫吸附测定（ELISA）
Enzyme linked immunosorbent assay (ELISA) for cytomegalovirus antibody in donor plasma.
作者信息
Ramskill S, Senior P, Barlow P, Tovey L A
机构信息
Leeds Regional Transfusion Centre.
出版信息
J Clin Pathol. 1988 Nov;41(11):1233-5. doi: 10.1136/jcp.41.11.1233.
Abstract
An adaptation of an enzyme immunoassay technique was developed to screen donor plasma for high titres of antibodies to cytomegalovirus (CMV). The technique uses microtitre plates treated with glutaraldehyde and coated with CMV antigen and an anti-IgG alkaline phosphatase conjugate to detect the captured antibody. Using an anti-CMV standard with a 1/64 titre by complement fixation, 34 (6.8%) of 500 sera were shown to have an antibody titre that was acceptable to the Blood Products Laboratory in England for anti-CMV immunoglobulin production.
摘要
开发了一种酶免疫测定技术的改良方法，用于筛查供体血浆中针对巨细胞病毒（CMV）的高滴度抗体。该技术使用经戊二醛处理并包被有CMV抗原的微量滴定板，以及抗IgG碱性磷酸酶结合物来检测捕获的抗体。使用补体结合法滴度为1/64的抗CMV标准品，500份血清中有34份（6.8%）的抗体滴度符合英国血液制品实验室用于生产抗CMV免疫球蛋白的要求。
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