Khedher Med Raâfet Ben, Khedher Saoussen Ben, Chaieb Ikbal, Tounsi Slim, Hammami Mohamed
Laboratory of Biochemistry, 'Nutrition, Functional Food and Vascular Health' Faculty of Medicine of Monastir, University of Monastir, 5000 Monastir, Tunisia.
Laboratory of Biopesticides, Centre of Biotechnology of Sfax, University of Sfax, P.O. Box 1177, 3018 Sfax, Tunisia.
EXCLI J. 2017 Mar 6;16:160-173. doi: 10.17179/excli2016-832. eCollection 2017.
The aim of this study is to evaluate the chemical composition, antioxidant, antimicrobial, insecticidal and allelopathic activities of Tunisia essential oil (SoEO). The SoEO was characterized by the presence of 49 components with camphor (25.14 %), α-thujone (18.83 %), 1,8-cineole (14.14 %), viridiflorol (7.98 %), β-thujone (4.46 %) and β-caryophyllene (3.30 %) as the major components, determined by gas chromatography-mass spectrometry. The level of antioxidant activity, determined by complementary tests, namely 2,2-diphenyl-1-picrylhydrazyl radical-scavenging (IC= 6.7 mg/mL), linoleic acid peroxidation (IC= 9.6 mg/mL) and ferric reducing assays (IC= 28.4 mg/mL), was relatively moderate. The SoEO was also screened for its antimicrobial activity. Good to moderate inhibitions were recorded for most of tested microorganisms. It also exhibited important insecticidal activity against larvae and adults with LC values of 55.99 and 97.43 µl/L air, respectively. The effect of the SoEO on seeds germination and growth showed different activities against radical and hypocotyl elongation of the tested species. These results suggest the potential use of the SoEO as natural antimicrobial preservative in cosmetic, pharmaceutical industry and in pest management.
本研究旨在评估突尼斯精油（SoEO）的化学成分、抗氧化、抗菌、杀虫和化感活性。通过气相色谱-质谱法测定，SoEO的特征在于含有49种成分，其中樟脑（25.14%）、α-侧柏酮（18.83%）、1,8-桉叶素（14.14%）、异绿花醇（7.98%）、β-侧柏酮（4.46%）和β-石竹烯（3.30%）为主要成分。通过2,2-二苯基-1-苦基肼自由基清除法（IC = 6.7 mg/mL）、亚油酸过氧化法（IC = 9.6 mg/mL）和铁还原法（IC = 28.4 mg/mL）等补充试验测定的抗氧化活性水平相对适中。还对SoEO的抗菌活性进行了筛选。对大多数测试微生物记录到良好至中等程度的抑制作用。它对幼虫和成虫也表现出重要的杀虫活性，LC值分别为55.99和97.43 μl/L空气。SoEO对种子萌发和生长的影响表明，其对受试物种的胚根和下胚轴伸长具有不同的活性。这些结果表明SoEO在化妆品、制药行业和害虫管理中作为天然抗菌防腐剂具有潜在用途。
