Janitabar-Darzi Simin, Rezaei Reza, Yavari Kamal
Radiopharmaceutical Research and Development Laboratory, Nuclear Science and Technology Research Institute, Tehran, Iran.
Department of biochemistry, Faculty of Science, Zanjan University, Zanjan, Iran.
Adv Pharm Bull. 2017 Apr;7(1):87-95. doi: 10.15171/apb.2017.011. Epub 2017 Apr 13.
Study on gold based therapeutic agents for cancer cells deracination has become under scrutiny in recent years owing to effective treatments are not available for rapidly progressive cancers. The aim of present study was to examine efficiency of radioactive Au/PAMAMG4 and non-radioactive Au/PAMAMG4 nancomposites against 4T1 and MCF7 breast cancer cell lines. The PAMAMG4 dendrimer was treated with the gold anions and then, the mixture was chemically reduced by NaBH. Prepared Au/PAMAMG4 was bombarded by thermal neutrons in the Tehran Research Reactor to Au/PAMAMG4 be produced. Prepared nanocomposites were characterized by means of FT-IR, 1H NMR, Zeta-potential measurements, TEM and EDX analyses. The radionuclidic purity of the Au/PAMAMG4 solution was determined using purity germanium (HPGe) spectroscopy and its stability in the presence of human serum was studied. In vitro studies were carried out to compare toxicity of PAMAMG4, Au/PAMAMG4 and Au/PAMAMG4 towards 4T1 and MCF7 cancerous cells and C2C12 normal cell lines. Characterization results exhibited that invitro agents, Au/PAMAMG4 and Au/PAMAMG4, were synthesized successfully. Cells viability after 24 h, 48 h, and 72 h incubation, using MTT assay showed that the toxicity of Au/PAMAMG4 is significantly superior in comparison with Au/PAMAMG4 and PAMAMG4. Furthermore, the toxicity of Au/PAMAMG4 was higher on cancerous cells especially in higher level of concentrations after 72 hours (P<0.05). In the current study, the preparation of Au/PAMAMG4 and Au/PAMAMG4 is described and the cytotoxic properties of them against the MCF7, 4T1 cancerous cells and C2C12 normal cells were evaluated using MTT assay.
由于目前尚无有效治疗快速进展性癌症的方法，近年来，关于用于根除癌细胞的金基治疗剂的研究备受关注。本研究的目的是检测放射性Au/PAMAMG4和非放射性Au/PAMAMG4纳米复合材料对4T1和MCF7乳腺癌细胞系的疗效。用金阴离子处理PAMAMG4树枝状大分子，然后用NaBH对混合物进行化学还原。制备的Au/PAMAMG4在德黑兰研究堆中用热中子轰击以产生Au/PAMAMG4。通过傅里叶变换红外光谱（FT-IR）、核磁共振氢谱（1H NMR）、zeta电位测量、透射电子显微镜（TEM）和能谱分析（EDX）对制备的纳米复合材料进行表征。用高纯锗（HPGe）光谱法测定Au/PAMAMG4溶液的放射性核素纯度，并研究其在人血清存在下的稳定性。进行体外研究以比较PAMAMG4、Au/PAMAMG4和Au/PAMAMG4对4T1和MCF7癌细胞以及C2C12正常细胞系的毒性。表征结果表明，成功合成了体外制剂Au/PAMAMG4和Au/PAMAMG4。使用MTT法检测孵育24小时、48小时和72小时后的细胞活力，结果表明Au/PAMAMG4的毒性明显优于Au/PAMAMG4和PAMAMG4。此外，Au/PAMAMG4对癌细胞的毒性更高，尤其是在72小时后浓度较高时（P<0.05）。在本研究中，描述了Au/PAMAMG4和Au/PAMAMG4的制备方法，并使用MTT法评估了它们对MCF7、4T1癌细胞和C2C12正常细胞的细胞毒性。
