Department of Organic and Biochemistry, Faculty of Chemistry, University of Tabriz, Tabriz, Iran; Research Institute of Bioscience and Biotechnology, University of Tabriz, Tabriz, Iran; University of Tabriz, Tabriz, 5156917511, Iran.
Department of Organic and Biochemistry, Faculty of Chemistry, University of Tabriz, Tabriz, Iran.
Photodiagnosis Photodyn Ther. 2021 Jun;34:102243. doi: 10.1016/j.pdpdt.2021.102243. Epub 2021 Mar 4.
Photothermal therapy (PTT) procedure is anticipated as a new generation of cancer therapy techniques. With this in mind, in this work, an effective drug-free approach was developed to kill MCF7 breast cancer cells using PTT. A novel biocompatible nanocomposite as a PTT transducer was prepared from the in situ phytosynthesis of gold nanoparticles (Au NPs) in the presence of perlite as a platform and extract of Allium Fistulosum L. as a stabilizing and reducing agent (Au/perlite NC). The common characterization techniques such as Fourier transform infrared (FT-IR), zeta potential, dynamic light scattering (DLS), X-ray diffraction (XRD), ultraviolet-visible (UV-vis), scanning electron microscopy (SEM), and energy-dispersive X-ray (EDX) were used to approve the synthesis of Au/perlite NC. The potential of the synthesized NCs on ROS generating and antioxidant activity was assessed by DPPH. In the following, the PTT efficacy of the Au/perlite NC on the destruction of MCF-7 breast cancer cells was assessed in vitro via the cell cycle, cell viability, and DAPI staining assays. The DPPH assay results showed that Au/perlite NC had a radicals scavenging capacity of about 41.47 % in 30 min. Cellular uptake results indicated a significant cell uptake after 1.5 h exposure with Au/perlite NC. Interestingly, cell death was increased dramatically by increasing irradiation time from 6 to 10 min. Cell viability assay revealed that the maximum number of cell death is around 50 % which was observed in the presence of Au/perlite NC by irradiation time of 10 min. Cell cycle results showed that the maximum amount of apoptotic cells (85 %) was observed in Au/perlite NC treatment group received laser irradiation for 10 min. The outcomes demonstrated that the Au/perlite NC can be used as a new drug-free and efficient agent for PTT of breast cancer cells without any concern cytotoxicity.
光热疗法（PTT）被认为是新一代癌症治疗技术。有鉴于此，本工作开发了一种使用 PTT 有效杀死 MCF7 乳腺癌细胞的无药物方法。通过在辉绿岩存在的情况下原位合成金纳米粒子（Au NPs），并使用葱属植物提取物作为稳定剂和还原剂（Au/perlite NC），制备了一种新型的生物相容性纳米复合材料作为 PTT 换能器。傅里叶变换红外（FT-IR）、zeta 电位、动态光散射（DLS）、X 射线衍射（XRD）、紫外可见（UV-vis）、扫描电子显微镜（SEM）和能谱（EDX）等常用表征技术用于证实 Au/perlite NC 的合成。通过 DPPH 评估了合成 NCs 对 ROS 生成和抗氧化活性的潜力。接下来，通过细胞周期、细胞活力和 DAPI 染色测定法评估了 Au/perlite NC 在体外对 MCF-7 乳腺癌细胞破坏的 PTT 功效。DPPH 测定结果表明，Au/perlite NC 在 30 分钟内具有约 41.47%的自由基清除能力。细胞摄取结果表明，在 Au/perlite NC 暴露 1.5 小时后，细胞摄取显著增加。有趣的是，随着照射时间从 6 分钟增加到 10 分钟，细胞死亡急剧增加。细胞活力测定表明，在 Au/perlite NC 存在下，照射时间为 10 分钟时，细胞死亡数最多约为 50%。细胞周期结果表明，在 Au/perlite NC 处理组中，观察到最大数量的凋亡细胞（85%）在接受激光照射 10 分钟时。结果表明，Au/perlite NC 可作为一种新的无药物且高效的 PTT 试剂，用于治疗乳腺癌细胞，而不会引起任何细胞毒性问题。
