Paupière Marine J, Müller Florian, Li Hanjing, Rieu Ivo, Tikunov Yury M, Visser Richard G F, Bovy Arnaud G
Plant Breeding, Wageningen University and Research Centre, PO Box 386, 6700 AJ, Wageningen, The Netherlands.
Molecular Plant Physiology, Institute for Water and Wetland Research, Radboud University, Heyendaalseweg 135, 6525 AJ, Nijmegen, The Netherlands.
Plant Reprod. 2017 Jun;30(2):81-94. doi: 10.1007/s00497-017-0301-6. Epub 2017 May 16.
Pollen development metabolomics. Developing pollen is among the plant structures most sensitive to high temperatures, and a decrease in pollen viability is often associated with an alteration of metabolite content. Most of the metabolic studies of pollen have focused on a specific group of compounds, which limits the identification of physiologically important metabolites. To get a better insight into pollen development and the pollen heat stress response, we used a liquid chromatography-mass spectrometry platform to detect secondary metabolites in pollen of tomato (Solanum lycopersicum L.) at three developmental stages under control conditions and after a short heat stress at 38 °C. Under control conditions, the young microspores accumulated a large amount of alkaloids and polyamines, whereas the mature pollen strongly accumulated flavonoids. The heat stress treatment led to accumulation of flavonoids in the microspore. The biological role of the detected metabolites is discussed. This study provides the first untargeted metabolomic analysis of developing pollen under a changing environment that can serve as reference for further studies.
花粉发育代谢组学。发育中的花粉是植物结构中对高温最为敏感的部分之一，花粉活力的下降通常与代谢物含量的改变有关。大多数关于花粉的代谢研究都集中在特定的一类化合物上，这限制了对生理上重要代谢物的识别。为了更好地了解花粉发育和花粉热应激反应，我们使用液相色谱 - 质谱平台，检测了在对照条件下以及在38°C短时间热应激后，番茄（Solanum lycopersicum L.）花粉在三个发育阶段的次生代谢物。在对照条件下，幼嫩小孢子积累了大量生物碱和多胺，而成熟花粉则大量积累黄酮类化合物。热应激处理导致小孢子中黄酮类化合物的积累。本文讨论了所检测到的代谢物的生物学作用。这项研究首次对变化环境下发育中的花粉进行了非靶向代谢组学分析，可为进一步研究提供参考。
