Yeh Ching-Hua, Chen Dennis, Aghdasi Bayan, Xiao Li, Ding Mengmeng, Jin Li, Li Xudong
a Department of Orthopaedic Surgery , University of Virginia , Charlottesville , VA , USA.
b Centre for Infectious Disease and Cancer Research , Kaohsiung Medical University , Kaohsiung , Taiwan.
Connect Tissue Res. 2018 Mar;59(2):191-200. doi: 10.1080/03008207.2017.1330333. Epub 2017 Jun 8.
Intervertebral disc degeneration is a major cause of back pain. Novel therapies for prevention or reversal of disc degeneration are needed. It is desirable for potential therapies to target both inflammation and matrix degeneration.
The combined regenerative potential of link protein N-terminal peptide (LN) and fullerol on annulus fibrosus (AF) cells was evaluated in a 3D culture model.
Interleukin-1α (IL-1α)-induced AF cell degeneration was counteracted by fullerol, LN, and fullerol + LN, with the latter having the greatest effect on matrix production as evaluated by real-time polymerase chain reaction and glycosaminoglycan assay. IL-1α-induced increases in pro-inflammatory mediators (interleukin-6 and cyclooxygenase-2) and matrix metalloproteinases (MMP-1, -2, -9, and -13) were also counteracted by fullerol and LN.
Our data demonstrate that LN and fullerol individually, and in combination, promote matrix production and have anti-inflammatory and anti-catabolic effects on AF cells.
椎间盘退变是背痛的主要原因。需要有预防或逆转椎间盘退变的新疗法。理想的潜在疗法应同时针对炎症和基质退变。
在三维培养模型中评估连接蛋白N端肽（LN）和富勒醇对纤维环（AF）细胞的联合再生潜力。
富勒醇、LN以及富勒醇+LN可对抗白细胞介素-1α（IL-1α）诱导的AF细胞退变，通过实时聚合酶链反应和糖胺聚糖测定评估，后者对基质产生的影响最大。富勒醇和LN也可对抗IL-1α诱导的促炎介质（白细胞介素-6和环氧化酶-2）和基质金属蛋白酶（MMP-1、-2、-9和-13）的增加。
我们的数据表明，LN和富勒醇单独及联合使用均可促进基质产生，并对AF细胞具有抗炎和抗分解代谢作用。
