二次谐波成像的生物学应用。
Biological applications of second harmonic imaging.
作者信息
Cox Guy
机构信息
Australian Centre for Microscopy and Microanalysis, University of Sydney, NSW, 2006, Australia.
出版信息
Biophys Rev. 2011 Sep;3(3):131. doi: 10.1007/s12551-011-0052-9. Epub 2011 Jul 20.
Abstract
Second Harmonic Generation (SHG) microscopy dates back to 1974, but effective biological use of the technique has a history of barely 10 years. It is now widely used to image collagen in many different applications, and is becoming useful for imaging myosin and some polysaccharides. A separate line on research has focussed on SHG dyes, which can provide high-speed indication of membrane potential and are now in use in neurobiology. This review looks at the progress to date in these different fields.
摘要
二次谐波产生（SHG）显微镜技术可追溯到1974年，但该技术在生物学上的有效应用历史却只有短短10年。如今，它在许多不同的应用中被广泛用于对胶原蛋白进行成像，并且在对肌球蛋白和一些多糖进行成像方面也变得越来越有用。另一项研究方向聚焦于SHG染料，其可提供膜电位的高速指示，目前已在神经生物学中得到应用。本文综述了这些不同领域迄今为止取得的进展。
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本文引用的文献
1
Quantitative analysis of collagen fiber organization in injured tendons using Fourier transform-second harmonic generation imaging.
Opt Express. 2010 Nov 22;18(24):24983-93. doi: 10.1364/OE.18.024983.
2
Pulse-modulated second harmonic imaging microscope quantitatively demonstrates marked increase of collagen in tumor after chemotherapy.
J Biomed Opt. 2010 Sep-Oct;15(5):056016. doi: 10.1117/1.3497565.
3
Probing myosin structural conformation in vivo by second-harmonic generation microscopy.
Proc Natl Acad Sci U S A. 2010 Apr 27;107(17):7763-8. doi: 10.1073/pnas.0914782107. Epub 2010 Apr 12.
4
Second harmonic generation imaging and Fourier transform spectral analysis reveal damage in fatigue-loaded tendons.
Ann Biomed Eng. 2010 May;38(5):1741-51. doi: 10.1007/s10439-010-9976-7. Epub 2010 Mar 16.
5
Alterations of the extracellular matrix in ovarian cancer studied by Second Harmonic Generation imaging microscopy.
BMC Cancer. 2010 Mar 11;10:94. doi: 10.1186/1471-2407-10-94.
6
Fibrillar collagen scoring by second harmonic microscopy: a new tool in the assessment of liver fibrosis.
J Hepatol. 2010 Mar;52(3):398-406. doi: 10.1016/j.jhep.2009.12.009. Epub 2010 Jan 17.
7
Fibro-C-Index: comprehensive, morphology-based quantification of liver fibrosis using second harmonic generation and two-photon microscopy.
J Biomed Opt. 2009 Jul-Aug;14(4):044013. doi: 10.1117/1.3183811.
8
Second-harmonic generation and two-photon-excited autofluorescence microscopy of cardiomyocytes: quantification of cell volume and myosin filaments.
J Biomed Opt. 2008 Nov-Dec;13(6):064018. doi: 10.1117/1.3027970.
9
Nonlinear optical imaging of cellular processes in breast cancer.
Microsc Microanal. 2008 Dec;14(6):532-48. doi: 10.1017/S1431927608080884.
10
Second-harmonic generation imaging of membrane potential with photon counting.
Microsc Microanal. 2008 Dec;14(6):526-31. doi: 10.1017/S1431927608080811.
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