二次谐波成像的生物学应用。
Biological applications of second harmonic imaging.
作者信息
Cox Guy
机构信息
Australian Centre for Microscopy and Microanalysis, University of Sydney, NSW, 2006, Australia.
出版信息
Biophys Rev. 2011 Sep;3(3):131. doi: 10.1007/s12551-011-0052-9. Epub 2011 Jul 20.
Abstract
Second Harmonic Generation (SHG) microscopy dates back to 1974, but effective biological use of the technique has a history of barely 10 years. It is now widely used to image collagen in many different applications, and is becoming useful for imaging myosin and some polysaccharides. A separate line on research has focussed on SHG dyes, which can provide high-speed indication of membrane potential and are now in use in neurobiology. This review looks at the progress to date in these different fields.
摘要
二次谐波产生（SHG）显微镜技术可追溯到1974年，但该技术在生物学上的有效应用历史却只有短短10年。如今，它在许多不同的应用中被广泛用于对胶原蛋白进行成像，并且在对肌球蛋白和一些多糖进行成像方面也变得越来越有用。另一项研究方向聚焦于SHG染料，其可提供膜电位的高速指示，目前已在神经生物学中得到应用。本文综述了这些不同领域迄今为止取得的进展。
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