Schwarz Norbert Georg, Mertens Eva, Winter Doris, Maiga-Ascofaré Oumou, Dekker Denise, Jansen Stephanie, Tappe Dennis, Randriamampionona Njary, May Jürgen, Rakotozandrindrainy Raphael, Schmidt-Chanasit Jonas
Research group Infectious disease epidemiology, Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany.
German Center for Infection Research, Hamburg Borstel-Lübeck, Germany.
PLoS One. 2017 May 16;12(5):e0176708. doi: 10.1371/journal.pone.0176708. eCollection 2017.
It was previously reported that a malaria infection may interfere with the specificity of a commercial ELISA test against Zika virus (ZIKV). We analyzed 1,216 plasma samples from healthy, pregnant women collected in two sites in Madagascar in 2010 for ZIKV antibodies using a commercial ELISA and for Plasmodium infection by PCR. This screen revealed six putative ZIKV-positive samples by ELISA. These results could not be confirmed by indirect immunofluorescence assays or virus neutralization tests. Four of these six samples were also positive for P. falciparum. We noted that the frequency of malaria positivity was higher in ZIKV-ELISA positive samples (50% and 100% in the two study sites) than ZIKV-negative samples (17% and 10%, respectively), suggesting that malaria may have led to false ZIKV-ELISA positives.
此前有报道称，疟疾感染可能会干扰针对寨卡病毒（ZIKV）的商业酶联免疫吸附测定（ELISA）检测的特异性。我们使用商业ELISA对2010年在马达加斯加两个地点收集的1216份健康孕妇血浆样本进行寨卡病毒抗体检测，并通过聚合酶链反应（PCR）检测疟原虫感染情况。该筛查通过ELISA发现了6份疑似寨卡病毒阳性样本。这些结果无法通过间接免疫荧光测定或病毒中和试验得到证实。这6份样本中有4份恶性疟原虫检测也呈阳性。我们注意到，寨卡病毒ELISA阳性样本中疟疾阳性的频率（两个研究地点分别为50%和100%）高于寨卡病毒阴性样本（分别为17%和10%），这表明疟疾可能导致了寨卡病毒ELISA假阳性结果。
