Justino Allisson Benatti, Pereira Mariana Nunes, Peixoto Leonardo Gomes, Vilela Danielle Diniz, Caixeta Douglas Carvalho, de Souza Adriele Vieira, Teixeira Renata Roland, Silva Heitor Cappato Guerra, de Moura Francyelle Borges Rosa, Moraes Izabela Barbosa, Espindola Foued Salmen
Institute of Genetics and Biochemistry, Federal University of Uberlândia , Uberlândia, Brazil.
Institute of Biomedical Sciences, Federal University of Uberlândia , Uberlândia, Brazil.
J Agric Food Chem. 2017 Jun 7;65(22):4428-4438. doi: 10.1021/acs.jafc.7b01355. Epub 2017 May 25.
A polyphenol-enriched fraction from Annona crassiflora fruit peel (Ac-Pef) containing chlorogenic acid, (epi)catechin, procyanidin B2, and caffeoyl-glucoside was investigated against hepatic oxidative and nitrosative stress in streptozotocin-induced diabetic rats. Serum biochemical parameters, hepatic oxidative and nitrosative status, glutathione defense system analysis, and in silico assessment of absorption, distribution, metabolism, excretion, and toxicity (ADMET) of the main compounds of Ac-Pef were carried out. Ac-Pef treatment during 30 days decreased serum alanine aminotransferase, aspartate aminotransferase, and alkaline phosphatase activities, as well as hepatic lipid peroxidation, protein carbonylation and nitration, inducible nitric oxide synthase level, and activities and expressions of glutathione peroxidase, superoxide dismutase, and catalase. There were increases in antioxidant capacity, glutathione reductase activity, and reduced glutathione level. ADMET predictions of Ac-Pef compounds showed favorable absorption and distribution, with no hepatotoxicity. A. crassiflora fruit peel showed hepatoprotective properties, indicating a promising natural source of bioactive molecules for prevention and therapy of diabetes complications.
对番荔枝（Annona crassiflora）果皮中富含多酚的组分（Ac-Pef）进行了研究，该组分含有绿原酸、（表）儿茶素、原花青素B2和咖啡酰葡萄糖苷，用于考察其对链脲佐菌素诱导的糖尿病大鼠肝脏氧化应激和亚硝化应激的影响。测定了血清生化参数、肝脏氧化和亚硝化状态、谷胱甘肽防御系统分析以及对Ac-Pef主要化合物的吸收、分布、代谢、排泄和毒性（ADMET）的计算机模拟评估。30天的Ac-Pef治疗降低了血清丙氨酸氨基转移酶、天冬氨酸氨基转移酶和碱性磷酸酶活性，以及肝脏脂质过氧化、蛋白质羰基化和硝化、诱导型一氧化氮合酶水平，以及谷胱甘肽过氧化物酶、超氧化物歧化酶和过氧化氢酶的活性和表达。抗氧化能力、谷胱甘肽还原酶活性和还原型谷胱甘肽水平有所增加。Ac-Pef化合物的ADMET预测显示其具有良好的吸收和分布，且无肝毒性。番荔枝果皮具有肝脏保护特性，表明其是预防和治疗糖尿病并发症的有前景的生物活性分子天然来源。
