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全球翻译重编程是植物免疫调节的一个基本层面。

Global translational reprogramming is a fundamental layer of immune regulation in plants.

作者信息

Xu Guoyong, Greene George H, Yoo Heejin, Liu Lijing, Marqués Jorge, Motley Jonathan, Dong Xinnian

机构信息

Howard Hughes Medical Institute-Gordon and Betty Moore Foundation, Department of Biology, Duke University, Durham, North Carolina 27708, USA.

出版信息

Nature. 2017 May 25;545(7655):487-490. doi: 10.1038/nature22371. Epub 2017 May 17.

DOI:10.1038/nature22371
PMID:28514447
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5485861/
Abstract

In the absence of specialized immune cells, the need for plants to reprogram transcription to transition from growth-related activities to defence is well understood. However, little is known about translational changes that occur during immune induction. Using ribosome footprinting, here we perform global translatome profiling on Arabidopsis exposed to the microbe-associated molecular pattern elf18. We find that during this pattern-triggered immunity, translation is tightly regulated and poorly correlated with transcription. Identification of genes with altered translational efficiency leads to the discovery of novel regulators of this immune response. Further investigation of these genes shows that messenger RNA sequence features are major determinants of the observed translational efficiency changes. In the 5' leader sequences of transcripts with increased translational efficiency, we find a highly enriched messenger RNA consensus sequence, R-motif, consisting of mostly purines. We show that R-motif regulates translation in response to pattern-triggered immunity induction through interaction with poly(A)-binding proteins. Therefore, this study provides not only strong evidence, but also a molecular mechanism, for global translational reprogramming during pattern-triggered immunity in plants.

摘要

在缺乏专门免疫细胞的情况下，植物重新编程转录以从与生长相关的活动过渡到防御的必要性已得到充分理解。然而，对于免疫诱导过程中发生的翻译变化却知之甚少。利用核糖体印迹技术，我们在此对暴露于微生物相关分子模式elf18的拟南芥进行了全转录组分析。我们发现，在这种模式触发的免疫过程中，翻译受到严格调控，且与转录的相关性较差。对翻译效率发生改变的基因进行鉴定，从而发现了这种免疫反应的新型调节因子。对这些基因的进一步研究表明，信使RNA序列特征是观察到的翻译效率变化的主要决定因素。在翻译效率提高的转录本的5'前导序列中，我们发现了一个高度富集的信使RNA共有序列，即R基序，其主要由嘌呤组成。我们表明，R基序通过与多聚腺苷酸结合蛋白相互作用来调节对模式触发免疫诱导的翻译。因此，本研究不仅为植物模式触发免疫过程中的全局翻译重编程提供了有力证据也提供了分子机制。

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