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细胞骨架-膜纳米表面-形态拓扑关系作为 RBC 结构总紊乱的基本机制。

Topological Relationships Cytoskeleton-Membrane Nanosurface-Morphology as a Basic Mechanism of Total Disorders of RBC Structures.

机构信息

Laboratory of Biophysics of Cell Membranes under Critical State, Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology, V.A. Negovsky Research Institute of General Reanimatology, 107031 Moscow, Russia.

Department of Medical and Biological Physics, Sechenov First Moscow State Medical University (Sechenov University), 119991 Moscow, Russia.

出版信息

Int J Mol Sci. 2022 Feb 12;23(4):2045. doi: 10.3390/ijms23042045.

DOI:10.3390/ijms23042045
PMID:35216154
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8876224/
Abstract

The state of red blood cells (RBCs) and their functional possibilities depend on the structural organization of the membranes. Cell morphology and membrane nanostructure are compositionally and functionally related to the cytoskeleton network. In this work, the influence of agents (hemin, endogenous oxidation during storage of packed RBCs, ultraviolet (UV) radiation, temperature, and potential of hydrogen (pH) changes) on the relationships between cytoskeleton destruction, membrane nanostructure, and RBC morphology was observed by atomic force microscope. It was shown that the influence of factors of a physical and biochemical nature causes structural rearrangements in RBCs at all levels of organization, forming a unified mechanism of disturbances in relationships "cytoskeleton-membrane nanosurface-cell morphology". Filament ruptures and, consequently, large cytoskeleton pores appeared. The pores caused membrane topological defects in the form of separate grain domains. Increasing loading doses led to an increase in the number of large cytoskeleton pores and defects and their fusion at the membrane nanosurfaces. This caused the changes in RBC morphology. Our results can be used in molecular cell biology, membrane biophysics, and in fundamental and practical medicine.

摘要

红细胞（RBC）的状态及其功能可能性取决于膜的结构组织。细胞形态和膜纳米结构在组成和功能上与细胞骨架网络相关。在这项工作中，通过原子力显微镜观察了试剂（血红素、储存过程中红细胞的内源性氧化、紫外线（UV）辐射、温度和氢离子（pH）变化的潜能）对细胞骨架破坏、膜纳米结构和 RBC 形态之间关系的影响。结果表明，物理和生化性质因素的影响会在所有组织水平上引起 RBC 的结构重排，形成细胞骨架-膜纳米表面-细胞形态关系紊乱的统一机制。纤维断裂，因此，大的细胞骨架孔出现。这些孔在膜纳米表面上以单独的颗粒域的形式导致拓扑缺陷。增加加载剂量会导致大的细胞骨架孔和缺陷的数量增加，并在膜纳米表面上融合。这导致 RBC 形态的变化。我们的结果可用于分子细胞生物学、膜生物物理学以及基础和实用医学。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/08f2/8876224/220f3f4e64f1/ijms-23-02045-g008.jpg
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