Porat Yaara, Giladi Moshe, Schneiderman Rosa S, Blat Roni, Shteingauz Anna, Zeevi Einav, Munster Mijal, Voloshin Tali, Kaynan Noa, Tal Orna, Kirson Eilon D, Weinberg Uri, Palti Yoram
Preclinical Research Department, Novocure Ltd., Haifa, Israel.
Preclinical Research Department, Novocure Ltd., Haifa, Israel;
J Vis Exp. 2017 May 4(123):55820. doi: 10.3791/55820.
Tumor Treating Fields (TTFields) are an effective treatment modality delivered via the continuous, noninvasive application of low-intensity (1-3 V/cm), alternating electric fields in the frequency range of several hundred kHz. The study of TTFields in tissue culture is carried out using the TTFields in vitro application system, which allows for the application of electric fields of varying frequencies and intensities to ceramic Petri dishes with a high dielectric constant (Ɛ > 5,000). Cancerous cell lines plated on coverslips at the bottom of the ceramic Petri dishes are subjected to TTFields delivered in two orthogonal directions at various frequencies to facilitate treatment outcome tests, such as cell counts and clonogenic assays. The results presented in this report demonstrate that the optimal frequency of the TTFields with respect to both cell counts and clonogenic assays is 200 kHz for both ovarian and glioma cells.
肿瘤治疗电场（TTFields）是一种通过持续、无创施加低强度（1-3 V/cm）、频率在几百千赫兹范围内的交变电场来实现的有效治疗方式。在组织培养中对TTFields的研究是使用TTFields体外应用系统进行的，该系统允许将不同频率和强度的电场施加到具有高介电常数（Ɛ > 5,000）的陶瓷培养皿上。接种在陶瓷培养皿底部盖玻片上的癌细胞系会受到在两个正交方向上以不同频率施加的TTFields作用，以利于进行诸如细胞计数和克隆形成试验等治疗效果测试。本报告中呈现的结果表明，对于卵巢癌和胶质瘤细胞，在细胞计数和克隆形成试验方面，TTFields的最佳频率均为200 kHz。
