斑马鱼幼体性腺组织解剖
Dissection of Larval Zebrafish Gonadal Tissue.
作者信息
Wang Xinjian, Chen Sijie, Zhang Wei, Ren Yiyuan, Zhang Quan, Peng Gang
机构信息
Institutes of Brain Science, State Key Laboratory of Medical Neurobiology and Collaborative Innovation Center for Brain Science, Fudan University.
Institutes of Brain Science, State Key Laboratory of Medical Neurobiology and Collaborative Innovation Center for Brain Science, Fudan University;
出版信息
J Vis Exp. 2017 Apr 26(122):55294. doi: 10.3791/55294.
Although wild zebrafish possess a ZZ/ZW sex-determination system, domesticated zebrafish have lost the sex chromosome. They utilize a polygenic sex determination system, where several genes distributed throughout the genome collectively determine the sex identities of individual fish. Currently, the genes involved in regulating gonad development and how they work remain elusive. Normally, isolating gonadal tissue is the first step to examine the sex developmental processes. Here, we present a procedure to isolate gonadal tissue from 17 dpf (days post fertilization) and 25 dpf zebrafish larvae. The isolated gonadal tissue may be subsequently examined by morphology and gene expression profiling.
虽然野生斑马鱼拥有ZZ/ZW性别决定系统，但驯化后的斑马鱼已经失去了性染色体。它们采用多基因性别决定系统，基因组中分布的几个基因共同决定个体鱼的性别身份。目前，参与调节性腺发育的基因及其作用方式仍不清楚。通常，分离性腺组织是研究性别发育过程的第一步。在这里，我们介绍一种从受精后17天（dpf）和25 dpf的斑马鱼幼虫中分离性腺组织的方法。随后可以通过形态学和基因表达谱分析来检查分离出的性腺组织。
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