大鼠垂体后叶中神经肽Y（NPY）结合位点的定量放射自显影定位
Quantitative autoradiographic localization of neuropeptide Y (NPY) binding sites in rat posterior pituitary lobe.
作者信息
Saavedra J M, Cruciani R
机构信息
Laboratory of Clinical Science, National Institute of Mental Health, Bethesda, Maryland 20892.
出版信息
Cell Mol Neurobiol. 1988 Sep;8(3):333-8. doi: 10.1007/BF00711175.
Abstract
- We have localized and quantified neuropeptide Y (NPY) binding sites in the rat pituitary gland after incubation of tissue sections in the presence of 125I-Bolton-Hunter NPY followed by autoradiography, computerized microdensitometry, and comparison to 125I-standards. 2. In the rat, NPY binding sites are localized exclusively to the part of the posterior pituitary lobe closer to the pituitary stalk. No NPY binding sites could be found in the intermediate or the anterior pituitary lobes. 3. Our results suggest a role for NPY in the regulation of pituitary function and, in particular, that of the neural lobe.
摘要
- 我们将组织切片在125I-博尔顿-亨特神经肽Y存在的情况下孵育，然后进行放射自显影、计算机显微密度测定，并与125I标准品进行比较，从而对大鼠垂体中的神经肽Y（NPY）结合位点进行了定位和定量。2. 在大鼠中，NPY结合位点仅定位于垂体后叶靠近垂体柄的部分。在垂体中叶或前叶未发现NPY结合位点。3. 我们的结果表明NPY在垂体功能调节中发挥作用，特别是在神经叶的功能调节中。
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