膜融合茎与脂筏：爱恨交织的关系。
Membrane Fusion Stalks and Lipid Rafts: A Love-Hate Relationship.
作者信息
Risselada Herre Jelger
机构信息
Institute for Theoretical Physics, Georg-August-Universität, Goettingen, Germany; Chemical Department, Leibniz Institute of Surface Modification, Leipzig, Germany.
出版信息
Biophys J. 2017 Jun 20;112(12):2475-2478. doi: 10.1016/j.bpj.2017.04.031. Epub 2017 May 16.
Abstract
Coarse-grained molecular dynamics simulations are applied to explore the experimentally observed ability of the liquid-ordered (lo)/liquid-disordered (ld) phase boundary to facilitate viral membrane fusion. Surprisingly, a formed fusion stalk can be both attracted (i.e., stalkophilic) and repelled (i.e., stalkophobic) by the lo/ld phase boundary. The phase boundary becomes stalkophilic if the lo phase constituents have the larger negative spontaneous curvature. In such a case, location of the highly curved stalk near the less-ordered and thus (relatively) softer boundary region becomes energetically favorable. These insights may explain why modeled viral fusion occurs preferentially near the lo/ld phase boundary.
摘要
应用粗粒度分子动力学模拟来探究实验观察到的液-有序（lo）/液-无序（ld）相界促进病毒膜融合的能力。令人惊讶的是，形成的融合茎既可以被lo/ld相界吸引（即亲茎），也可以被排斥（即疏茎）。如果lo相成分具有更大的负自发曲率，则相界会变成亲茎的。在这种情况下，高度弯曲的茎位于较无序因而（相对）较软的边界区域附近在能量上变得有利。这些见解可能解释了为什么模拟的病毒融合优先发生在lo/ld相界附近。
相似文献
1
Membrane Fusion Stalks and Lipid Rafts: A Love-Hate Relationship.
Biophys J. 2017 Jun 20;112(12):2475-2478. doi: 10.1016/j.bpj.2017.04.031. Epub 2017 May 16.
2
Pair interactions among ternary DPPC/POPC/cholesterol mixtures in liquid-ordered and liquid-disordered phases.
Soft Matter. 2016 May 18;12(20):4557-61. doi: 10.1039/c6sm00345a.
3
Permeability of membranes in the liquid ordered and liquid disordered phases.
Nat Commun. 2019 Dec 9;10(1):5616. doi: 10.1038/s41467-019-13432-7.
4
Liquid-ordered microdomains in lipid rafts and plasma membrane of U-87 MG cells: a time-resolved fluorescence study.
Eur Biophys J. 2003 Jul;32(4):381-91. doi: 10.1007/s00249-003-0281-3. Epub 2003 Mar 6.
5
Line tension at lipid phase boundaries as driving force for HIV fusion peptide-mediated fusion.
Nat Commun. 2016 Apr 26;7:11401. doi: 10.1038/ncomms11401.
6
HIV gp41-mediated membrane fusion occurs at edges of cholesterol-rich lipid domains.
Nat Chem Biol. 2015 Jun;11(6):424-31. doi: 10.1038/nchembio.1800. Epub 2015 Apr 27.
7
A coarse-grained molecular dynamics investigation of the phase behavior of DPPC/cholesterol mixtures.
Chem Phys Lipids. 2015 Jan;185:88-98. doi: 10.1016/j.chemphyslip.2014.07.011. Epub 2014 Aug 1.
8
Boundary lipids of the nicotinic acetylcholine receptor: Spontaneous partitioning via coarse-grained molecular dynamics simulation.
Biochim Biophys Acta Biomembr. 2019 Apr 1;1861(4):887-896. doi: 10.1016/j.bbamem.2019.01.005. Epub 2019 Jan 18.
9
Polyunsaturated chains in asymmetric lipids disorder raft mixtures and preferentially associate with α-Synuclein.
Biochim Biophys Acta Biomembr. 2017 Apr;1859(4):529-536. doi: 10.1016/j.bbamem.2016.10.006. Epub 2016 Oct 11.
10
Polar Glycerolipids and Membrane Lipid Rafts.
Int J Mol Sci. 2024 Jul 30;25(15):8325. doi: 10.3390/ijms25158325.
引用本文的文献
1
Visualizing intermediate stages of viral membrane fusion by cryo-electron tomography.
Trends Biochem Sci. 2024 Oct;49(10):916-931. doi: 10.1016/j.tibs.2024.06.012. Epub 2024 Jul 24.
2
Antiviral activity of the host defense peptide piscidin 1: investigating a membrane-mediated mode of action.
Front Chem. 2024 Jun 26;12:1379192. doi: 10.3389/fchem.2024.1379192. eCollection 2024.
3
Dynamic Fusion of Nucleic Acid Functionalized Nano-/Micro-Cell-Like Containments: From Basic Concepts to Applications.
ACS Nano. 2023 Aug 22;17(16):15308-15327. doi: 10.1021/acsnano.3c04415. Epub 2023 Aug 7.
4
Cyclic lipopeptides as membrane fusion inhibitors against SARS-CoV-2: New tricks for old dogs.
Antiviral Res. 2023 Apr;212:105575. doi: 10.1016/j.antiviral.2023.105575. Epub 2023 Mar 2.
5
Correlation between the In-Plane Critical Behavior and Out-of-Plane Interaction of Ternary Lipid Membranes.
Membranes (Basel). 2022 Dec 21;13(1):6. doi: 10.3390/membranes13010006.
6
Modulating membrane fusion through the design of fusogenic DNA circuits and bilayer composition.
Soft Matter. 2022 Sep 28;18(37):7035-7044. doi: 10.1039/d2sm00863g.
7
Membrane Organization Strategies in Vesicular Antibiotic Delivery.
J Membr Biol. 2022 Oct;255(4-5):523-535. doi: 10.1007/s00232-021-00210-0. Epub 2022 Jan 11.
8
Fast bilayer-micelle fusion mediated by hydrophobic dipeptides.
Biophys J. 2021 Jun 1;120(11):2330-2342. doi: 10.1016/j.bpj.2021.04.012. Epub 2021 Apr 19.
9
Membrane Rafts: Portals for Viral Entry.
Front Microbiol. 2021 Feb 4;12:631274. doi: 10.3389/fmicb.2021.631274. eCollection 2021.
10
How proteins open fusion pores: insights from molecular simulations.
Eur Biophys J. 2021 Mar;50(2):279-293. doi: 10.1007/s00249-020-01484-3. Epub 2020 Dec 19.
本文引用的文献
1
Line tension at lipid phase boundaries as driving force for HIV fusion peptide-mediated fusion.
Nat Commun. 2016 Apr 26;7:11401. doi: 10.1038/ncomms11401.
2
High cholesterol obviates a prolonged hemifusion intermediate in fast SNARE-mediated membrane fusion.
Biophys J. 2015 Jul 21;109(2):319-29. doi: 10.1016/j.bpj.2015.06.022.
3
HIV gp41-mediated membrane fusion occurs at edges of cholesterol-rich lipid domains.
Nat Chem Biol. 2015 Jun;11(6):424-31. doi: 10.1038/nchembio.1800. Epub 2015 Apr 27.
4
Expansion of the fusion stalk and its implication for biological membrane fusion.
Proc Natl Acad Sci U S A. 2014 Jul 29;111(30):11043-8. doi: 10.1073/pnas.1323221111. Epub 2014 Jul 14.
5
Line-tension controlled mechanism for influenza fusion.
PLoS One. 2012;7(6):e38302. doi: 10.1371/journal.pone.0038302. Epub 2012 Jun 28.
6
A scissors mechanism for stimulation of SNARE-mediated lipid mixing by cholesterol.
Proc Natl Acad Sci U S A. 2009 Mar 31;106(13):5141-6. doi: 10.1073/pnas.0813138106. Epub 2009 Feb 27.
7
The molecular face of lipid rafts in model membranes.
Proc Natl Acad Sci U S A. 2008 Nov 11;105(45):17367-72. doi: 10.1073/pnas.0807527105. Epub 2008 Nov 5.
8
Mechanics of membrane fusion.
Nat Struct Mol Biol. 2008 Jul;15(7):675-83. doi: 10.1038/nsmb.1455.
9
Dependence of the energies of fusion on the intermembrane separation: optimal and constrained.
J Chem Phys. 2007 Aug 21;127(7):075102. doi: 10.1063/1.2766945.
10
Line tension at fluid membrane domain boundaries measured by micropipette aspiration.
Phys Rev Lett. 2007 May 18;98(20):208102. doi: 10.1103/PhysRevLett.98.208102. Epub 2007 May 16.
Zotero 插件