Bibi Fehmida, Alvi Sana Akhtar, Sawan Sara Ali, Yasir Muhammad, Sawan Ali, Jiman-Fatani Asif A, Azhar Esam I
Fehmida Bibi, Special Infectious Agents Unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah, 21589, Kingdom of Saudi Arabia.
Sana Akhtar Alvi, Special Infectious Agents Unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah, 21589, Kingdom of Saudi Arabia.
Pak J Med Sci. 2017 Mar-Apr;33(2):320-324. doi: 10.12669/pjms.332.12024.
() infection is cause of several gastrointestinal diseases in humans. Virulence genes of are associated with severity of disease and vary geographically. The aim of present study was to detect in formalin-fixed paraffin-embedded (FFPE) tissues and further investigate prevalence of A, and genotypes in from gastric cancer (GC) and gastric ulcer (GU) patients' biopsy samples.
We used FFPE tissues of 35 GC and 10 GU patients' biopsy samples. Using Polymerase Chain Reaction (PCR), detection of strain was performed by using specific primers targeting 16S rRNA and encodes for phosphoglucosamine mutase genes. We have identified different virulence genes of by PCR.
Of all the 45 samples tested, 20 GC and all 10 GU samples were positive for identification of using specific genes (16S rRNA and ). The prevalence of (100%) was significantly higher in GC as compared to GU (40%) samples. The rate of virulence genes was higher in both GU 8 (80%) and GC (100%).
Our study finds that and are most prominent genotypes and may play role in development of Gastric cancer.
（）感染是人类多种胃肠道疾病的病因。（）的毒力基因与疾病严重程度相关且存在地域差异。本研究的目的是在福尔马林固定石蜡包埋（FFPE）组织中检测（），并进一步调查来自胃癌（GC）和胃溃疡（GU）患者活检样本中的（）A、（）和（）基因型的流行情况。
我们使用了35例GC和10例GU患者活检样本的FFPE组织。采用聚合酶链反应（PCR），通过使用靶向16S rRNA和编码磷酸葡糖胺变位酶基因的特异性引物进行（）菌株的检测。我们通过PCR鉴定了（）的不同毒力基因。
在所有检测的45个样本中，20例GC样本和所有10例GU样本使用特异性基因（16S rRNA和（））鉴定（）呈阳性。与GU（40%）样本相比，（）在GC中的流行率（100%）显著更高。毒力基因（）在GU（80%）和GC（100%）中的比例均较高。
我们的研究发现（）和（）是最主要的基因型，可能在胃癌的发生中起作用。
