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用于人类肠道病毒的非同位素寡聚探针。

Nonisotopic oligomeric probes for the human enteroviruses.

作者信息

Rotbart H A, Eastman P S, Ruth J L, Hirata K K, Levin M J

机构信息

Department of Pediatrics, School of Medicine, University of Colorado 80262.

出版信息

J Clin Microbiol. 1988 Dec;26(12):2669-71. doi: 10.1128/jcm.26.12.2669-2671.1988.

DOI:10.1128/jcm.26.12.2669-2671.1988
PMID:2852677
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC266971/
Abstract

Oligonucleotide probes were prepared from highly conserved regions of human enteroviral genomes. These reagents were labeled with either 32P or alkaline phosphatase and were successfully used in blot assays to detect a wide variety of human enteroviruses, proving the potential utility of oligomeric sequences as pan-enteroviral probes. The availability of nonisotopic probes will ultimately make a hybridization assay for enteroviruses easier, shorter, and more adaptable for routine diagnostic laboratories.

摘要

寡核苷酸探针是从人肠道病毒基因组的高度保守区域制备的。这些试剂用32P或碱性磷酸酶标记，并成功用于印迹分析以检测多种人肠道病毒，证明了寡聚序列作为泛肠道病毒探针的潜在用途。非同位素探针的可用性最终将使肠道病毒的杂交检测更容易、更快捷，并且更适合常规诊断实验室。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4c53/266971/87d659a60c4d/jcm00084-0218-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4c53/266971/b4bca7c955c7/jcm00084-0218-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4c53/266971/87d659a60c4d/jcm00084-0218-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4c53/266971/b4bca7c955c7/jcm00084-0218-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4c53/266971/87d659a60c4d/jcm00084-0218-b.jpg

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本文引用的文献

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The clinical relevance of 'CSF viral culture'. A two-year experience with aseptic meningitis in Rochester, NY.“脑脊液病毒培养”的临床相关性。纽约罗切斯特地区无菌性脑膜炎的两年经验。
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Molecular detection and identification of enteroviruses using enzymatic amplification and nucleic acid hybridization.利用酶促扩增和核酸杂交技术对肠道病毒进行分子检测与鉴定。
J Clin Microbiol. 1990 May;28(5):843-50. doi: 10.1128/jcm.28.5.843-850.1990.
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Enzymatic RNA amplification of the enteroviruses.肠道病毒的酶促RNA扩增
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A structural model for the genome of echovirus 22.
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Nucleic acid detection systems for enteroviruses.肠道病毒核酸检测系统
Clin Microbiol Rev. 1991 Apr;4(2):156-68. doi: 10.1128/CMR.4.2.156.
9
Detection of enteroviral RNA in idiopathic dilated cardiomyopathy and other human cardiac tissues.在特发性扩张型心肌病及其他人体心脏组织中检测肠道病毒RNA。
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用于检测和鉴定A组柯萨奇病毒的酶联免疫吸附测定法。
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Use of subgenomic poliovirus DNA hybridization probes to detect the major subgroups of enteroviruses.使用亚基因组脊髓灰质炎病毒DNA杂交探针检测肠道病毒的主要亚组。
J Clin Microbiol. 1984 Dec;20(6):1105-8. doi: 10.1128/jcm.20.6.1105-1108.1984.
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J Clin Microbiol. 1985 Aug;22(2):220-4. doi: 10.1128/jcm.22.2.220-224.1985.