使用共沉淀剂增强AMPLICOR肠道病毒聚合酶链反应检测
Enhancement of the AMPLICOR enterovirus PCR test with a coprecipitant.
作者信息
Taggart E W, Byington C L, Hillyard D R, Robison J E, Carroll K C
机构信息
Associated Regional and University Pathologists Institute for Clinical and Experimental Pathology, University of Utah Health Sciences Center, Salt Lake City, Utah, USA.
出版信息
J Clin Microbiol. 1998 Nov;36(11):3408-9. doi: 10.1128/JCM.36.11.3408-3409.1998.
The incorporation of a commercially available coprecipitant into the AMPLICOR enterovirus PCR test specimen preparation enhanced the sensitivity and reproducibility of this assay. Fifty-five previously tested archived cerebrospinal fluids (CSF) specimens were tested in a blind study in duplicate with and without Pellet Paint coprecipitant (Novagen, Inc., Madison, Wis.). Of these specimens, 26 had previously been determined to be positive and 29 had previously been determined to be negative. All previously positive CSF specimens were positive when Pellet Paint was used and only 18 were positive without Pellet Paint. No previously negative specimens were positive on repeat testing with or without Pellet Paint. The background signal was not affected by the addition of Pellet Paint. These data support the utility of a coprecipitant in minimizing false-negative results.
将一种市售的共沉淀剂加入到AMPLICOR肠道病毒PCR检测样本制备过程中，可提高该检测方法的灵敏度和重复性。在一项盲法研究中，对55份之前检测过的存档脑脊液（CSF）样本进行了一式两份的检测，分别添加和不添加Pellet Paint共沉淀剂（Novagen公司，威斯康星州麦迪逊）。在这些样本中，之前已确定26份为阳性，29份为阴性。使用Pellet Paint时，所有之前呈阳性的CSF样本均为阳性，而不使用Pellet Paint时只有18份呈阳性。无论是否添加Pellet Paint，之前呈阴性的样本在重复检测时均未呈阳性。添加Pellet Paint不影响背景信号。这些数据支持了共沉淀剂在减少假阴性结果方面的作用。
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