Department of Prosthodontics, Ninth People's Hospital affiliated to Shanghai Jiao Tong University, School of Medicine, 639 Zhizaoju Road, Shanghai, 200011, China.
Oral Bioengineering and regenerative medicine Lab, Shanghai Research Institute of Stomatology, Ninth People's Hospital Affiliated to Shanghai Jiao Tong University, School of Medicine, Shanghai Key Laboratory of Stomatology, 639 Zhizaoju Road, Shanghai, 200011, China.
Sci Rep. 2017 May 19;7(1):2175. doi: 10.1038/s41598-017-02053-z.
The low in vivo survival rate of scaffold-seeded cells is still a challenge in stem cell-based bone regeneration. This study seeks to use a silk hydrogel to deliver more stem cells into a bone defect area and prolong the viability of these cells after implantation. Rat bone marrow stem cells were mingled with silk hydrogels at the concentrations of 1.0 × 10/mL, 1.0 × 10/mL and 1.0 × 10/mL before gelation, added dropwise to a silk scaffold and applied to a rat calvarial defect. A cell tracing experiment was included to observe the preservation of cell viability and function. The results show that the hydrogel with 1.0 × 10/mL stem cells exhibited the best osteogenic effect both in vitro and in vivo. The cell-tracing experiment shows that cells in the 1.0 × 10 group still survive and actively participate in new bone formation 8 weeks after implantation. The strategy of pre-mingling stem cells with the hydrogel had the effect of delivering more stem cells for bone engineering while preserving the viability and functions of these cells in vivo.
支架细胞体内存活率低仍然是干细胞骨再生面临的挑战。本研究旨在利用丝素水凝胶将更多的干细胞输送到骨缺损区域，并延长植入后这些细胞的活力。将浓度为 1.0×10/mL、1.0×10/mL 和 1.0×10/mL 的骨髓干细胞与丝素水凝胶在凝胶前混合，滴加到丝素支架上，并应用于大鼠颅骨缺损。进行细胞示踪实验以观察细胞活力和功能的保存情况。结果表明，浓度为 1.0×10/mL 干细胞的水凝胶在体外和体内均表现出最佳的成骨效果。细胞示踪实验表明，植入 8 周后，1.0×10 组的细胞仍然存活并积极参与新骨形成。将干细胞与水凝胶预先混合的策略具有输送更多干细胞进行骨工程的效果，同时在体内保持这些细胞的活力和功能。
