Owen Charlie, Patron Nicola J, Huang Ancheng, Osbourn Anne
Department of Metabolic Biology, John Innes Centre, Norwich Research Park, Norwich NR4 7UH, UK.
Engineering Biology, The Earlham Institute, Norwich Research Park, Norwich NR4 7UZ, UK.
Curr Opin Chem Biol. 2017 Oct;40:24-30. doi: 10.1016/j.cbpa.2017.04.015. Epub 2017 May 17.
Advances in DNA sequencing and synthesis technologies in the twenty-first century are now making it possible to build large-scale pipelines for engineering plant natural product pathways into heterologous production species using synthetic biology approaches. The ability to decode the chemical potential of plants by sequencing their transcriptomes and/or genomes and to then use this information as an instruction manual to make drugs and other high-value chemicals is opening up new routes to harness the vast chemical diversity of the Plant Kingdom. Here we describe recent progress in methods for pathway discovery, DNA synthesis and assembly, and expression of engineered pathways in heterologous hosts. We also highlight the importance of standardization and the challenges associated with dataset integration in the drive to build a systematic framework for effective harnessing of plant metabolic diversity.
21世纪DNA测序和合成技术的进步，使得利用合成生物学方法构建大规模管道成为可能，这些管道可用于将植物天然产物途径工程化到异源生产物种中。通过对植物转录组和/或基因组进行测序来解码植物的化学潜能，然后将这些信息用作制作药物和其他高价值化学品的操作指南，这种能力正在开辟新途径，以利用植物王国的巨大化学多样性。在此，我们描述了在途径发现、DNA合成与组装以及工程化途径在异源宿主中的表达等方法方面的最新进展。我们还强调了标准化的重要性，以及在构建有效利用植物代谢多样性的系统框架过程中与数据集整合相关的挑战。
