Teilmann Anne C, Thomsen Morten B, Ihms Elizabeth A, Pate Nathan, Hau Jann, Abelson Klas
1 Department of Experimental Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.
2 Department of Biomedical Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.
Lab Anim. 2018 Feb;52(1):17-28. doi: 10.1177/0023677217706724. Epub 2017 May 22.
Catheterization of laboratory mice is commonly performed in biomedical research to infuse substances and for blood sampling. One approach is to catheterize the right common carotid artery and advance the catheter until the tip is positioned in the aorta or the proximal brachiocephalic trunk. Owing to the small body size of the mouse, a catheter tends to occupy a great part of even the larger vessel lumens, and this may increase vascular resistance with potential pathophysiological impacts on the heart. The present study compared cardiac function of catheterized mice, with catheter tip placement in the brachiocephalic trunk, with sham-operated mice and non-operated control mice. During four weeks post-catheterization, M-mode echocardiography measurements of the thickness of the left ventricular anterior wall, left ventricular inner diameter and the thickness of the left ventricular posterior wall were performed. The left ventricular volume, ejection fraction and fractional shortening were calculated. Moreover, aortic recordings of the thickness of the medial and lateral walls as well as the inner diameter were measured. Terminally, histological analysis of the hearts was conducted, and body weights and heart weights were compared between groups. No effects on echocardiography parameters, histology, body weights or cardiac weights could be found between groups. In the present study, implantation of a carotid catheter with catheter tip placement in the proximal brachiocephalic trunk had minimal influence on cardiac and aortic physiology and did not induce significant cardiac changes.
在生物医学研究中，对实验小鼠进行导管插入术通常是为了注入物质和采集血液样本。一种方法是将导管插入右侧颈总动脉，并将导管推进，直到尖端位于主动脉或近端头臂干。由于小鼠体型小，即使是较大的血管腔，导管也往往会占据很大一部分，这可能会增加血管阻力，对心脏产生潜在的病理生理影响。本研究比较了导管尖端置于头臂干的插管小鼠与假手术小鼠和未手术对照小鼠的心脏功能。在插管后四周内，采用M型超声心动图测量左心室前壁厚度、左心室内径和左心室后壁厚度。计算左心室容积、射血分数和缩短分数。此外，还测量了主动脉内侧壁和外侧壁厚度以及内径。最后，对心脏进行组织学分析，并比较各组之间的体重和心脏重量。各组之间在超声心动图参数、组织学、体重或心脏重量方面均未发现影响。在本研究中，将导管尖端置于近端头臂干的颈动脉导管植入对心脏和主动脉生理的影响最小，且未引起明显的心脏变化。
