Synthetic Biology Center, Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.
Nat Chem Biol. 2017 Jul;13(7):706-708. doi: 10.1038/nchembio.2390. Epub 2017 May 22.
Optogenetic tools use colored light to rapidly control gene expression in space and time. We designed a genetically encoded system that gives Escherichia coli the ability to distinguish between red, green, and blue (RGB) light and respond by changing gene expression. We use this system to produce 'color photographs' on bacterial culture plates by controlling pigment production and to redirect metabolic flux by expressing CRISPRi guide RNAs.
光遗传学工具利用有色光在空间和时间上快速控制基因表达。我们设计了一种遗传编码系统，使大肠杆菌能够区分红、绿、蓝（RGB）光，并通过改变基因表达来做出响应。我们使用该系统通过控制色素产生来在细菌培养板上生成“彩色照片”，并通过表达 CRISPRi 引导 RNA 来重新引导代谢通量。
