用于细胞内超分辨率显微镜的生物正交双荧光硅罗丹明探针。
Bioorthogonal double-fluorogenic siliconrhodamine probes for intracellular super-resolution microscopy.
作者信息
Kozma E, Estrada Girona G, Paci G, Lemke E A, Kele P
机构信息
"Lendület" Chemical Biology Research Group, Institute of Organic Chemistry, Research Centre for Natural Sciences, Hungarian Academy of Sciences, Magyar tudósok krt. 2, 1117 Budapest, Hungary.
Structural and Computational Biology Unit, Cell Biology and Biophysics Unit, European Molecular Biology Laboratory, Meyerhofstrasse 1, Heidelberg, D-69117, Germany.
出版信息
Chem Commun (Camb). 2017 Jun 20;53(50):6696-6699. doi: 10.1039/c7cc02212c.
A series of double-fluorogenic siliconrhodamine probes were synthesized. These tetrazine-functionalized, membrane-permeable labels allowed site-specific bioorthogonal tagging of genetically manipulated intracellular proteins and subsequent imaging using super-resolution microscopy.
合成了一系列双荧光硅罗丹明探针。这些经四嗪功能化、可透过细胞膜的标记物能够对基因操控的细胞内蛋白质进行位点特异性生物正交标记，并随后使用超分辨率显微镜进行成像。
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