Department of Chemistry and Chemical Engineering, Beijing Forestry University, Beijing 100083, China.
Int J Biol Macromol. 2013 Apr;55:282-8. doi: 10.1016/j.ijbiomac.2013.01.025. Epub 2013 Jan 25.
Enzymatic hydrolysis of Gleditsia sinensis gum was performed to produce manno-oligosaccharides having functional applications as dietary fiber and prebiotics. The optimum hydrolysis conditions, including enzyme loading, temperature and time, from response surface methodology were 8.1 U/g, 57.4 °C and 34.1 h, respectively. The yield of DP 1-5 oligosaccharides was 75.9% (29.1 g/L). The Michaelis-Menten kinetics and molecular weight distribution were determined. The obtained oligosaccharides were further separated by HPLC and SEC, and the galactose distribution of G. sinensis gum was elucidated. Results indicated that G. sinensis gum has potential to produce value-added oligosaccharides in food industries.
采用酶解法从皂荚中制备具有膳食纤维和益生元功能的甘露低聚糖。通过响应面法优化了酶解条件，包括酶用量、温度和时间，分别为 8.1 U/g、57.4°C 和 34.1 h。低聚糖中 DP1-5 的产率为 75.9%（29.1 g/L）。测定了米氏动力学和分子量分布。进一步通过 HPLC 和 SEC 对获得的低聚糖进行分离，并阐明了皂荚胶的半乳糖分布。结果表明，皂荚胶在食品工业中有潜力生产具有附加值的低聚糖。
