Liu Jinghua, Huang Qing
Institute of Technical Biology and Agriculture Engineering, Hefei Institutes of Physical Science, Key Lab of Ion-Beam Bioengineering, Chinese Academy of Sciences, Hefei, China School of Life Science, University of Science and Technology of China, Hefei, China.
Institute of Technical Biology and Agriculture Engineering, Hefei Institutes of Physical Science, Key Lab of Ion-Beam Bioengineering, Chinese Academy of Sciences, Hefei, China School of Life Science, University of Science and Technology of China, Hefei, China School of Nuclear Science and Technology, University of Science and Technology of China, Hefei, China
Appl Spectrosc. 2016 Oct 1;70(10):1639-1648. doi: 10.1177/0003702816645605. Epub 2016 Jun 13.
Haematococcus pluvialis has promising applications owing to its ability to accumulate astaxanthin under stress conditions. In order to acquire higher astaxanthin productivity from H. pluvialis, it is critical not only to develop efficient mutagenesis techniques, but also to establish rapid and effective screening methods which are highly demanded in current research and application practice. In this work, we therefore attempted to develop a new approach to screening the astaxanthin-hyperproducing strains based on spectroscopic tools. Using Fourier transform infrared (FT-IR) and Raman microspectroscopy, we have achieved rapid and quantitative analysis of the algal cells in terms of astaxanthin, β-carotene, proteins, lipids, and carbohydrates. In particular, we have found that the ratio of the IR absorption band at 1740 cm to the band at 1156 cm can be utilized for identifying astaxanthin-hyperproducing strains. This work may therefore open a new avenue for developing high-throughput screening methods necessary for the microbial mutant breeding industry.
雨生红球藻因其在胁迫条件下积累虾青素的能力而具有广阔的应用前景。为了从雨生红球藻中获得更高的虾青素产量，不仅要开发高效的诱变技术，还要建立快速有效的筛选方法，这在当前的研究和应用实践中是非常必要的。因此，在这项工作中，我们试图开发一种基于光谱工具筛选高产虾青素菌株的新方法。利用傅里叶变换红外（FT-IR）和拉曼显微光谱，我们实现了对藻细胞中虾青素、β-胡萝卜素、蛋白质、脂质和碳水化合物的快速定量分析。特别是，我们发现1740 cm处的红外吸收带与1156 cm处的吸收带的比值可用于鉴定高产虾青素菌株。因此，这项工作可能为开发微生物突变育种行业所需的高通量筛选方法开辟一条新途径。
