Department of Materials Science and Engineering, Korea University , 145 Anam-ro, Seongbuk-gu, Seoul, 02841, Korea.
ACS Appl Mater Interfaces. 2017 Jun 14;9(23):19591-19600. doi: 10.1021/acsami.7b03308. Epub 2017 Jun 1.
The goal of this study is to fabricate a stable plasma filtration membrane with antibiofouling properties via an electrospinning process. To this end, a random-type copolymer consisting of zwitterionic phosphorylcholine (PC) groups and ultraviolet (UV)-cross-linkable phenyl azide groups was synthesized. The zwitterionic PC group provides antibiofouling properties, and the phenyl azide group enables the stable maintenance of the fibrous nanostructure of hydrophilic zwitterion polymers in aqueous medium via a simple UV curing process. To demonstrate the antibiofouling nature of the PC group, a polymer without antibiofouling PC groups was also prepared for comparison. The successful synthesis of the random-type copolymers containing phenyl azide groups was proven by H nuclear magnetic resonance and Fourier transform infrared spectroscopy, and the fibrous structure of the prepared membranes was observed by field emission scanning electron microscopy. The antibiofouling properties were analyzed by fluorescein isothiocyanate-labeled bovine serum albumin adsorption and platelet adhesion tests. The experimental results show that membranes containing zwitterionic PC groups exhibited obvious decreases in platelet adhesion and protein adsorption. Platelet-rich plasma solution was filtered using the prepared membranes to test their filtration properties. The sequential filtration process removed 80% and almost 98% of the platelets. This finding confirmed that the membrane retained its blood-inert biomaterial surface in a complex medium that included blood plasma and platelets.
本研究旨在通过静电纺丝工艺制备具有抗生物污染性能的稳定等离子体过滤膜。为此，合成了一种由两性离子磷酸胆碱（PC）基团和紫外（UV）可交联苯叠氮基团组成的无规共聚物。两性离子 PC 基团提供抗生物污染性能，苯叠氮基团通过简单的 UV 固化工艺使亲水性两性离子聚合物的纤维纳米结构稳定维持。为了证明 PC 基团的抗生物污染性质，还制备了不含抗生物污染 PC 基团的聚合物进行比较。通过 H 核磁共振和傅里叶变换红外光谱证明了含苯叠氮基团的无规共聚物的成功合成，并通过场发射扫描电子显微镜观察了所制备的膜的纤维结构。通过荧光素异硫氰酸酯标记牛血清白蛋白吸附和血小板黏附试验分析了抗生物污染性能。实验结果表明，含有两性离子 PC 基团的膜表现出明显降低的血小板黏附和蛋白质吸附。使用制备的膜过滤富含血小板的血浆溶液以测试其过滤性能。连续过滤过程去除了 80%和近 98%的血小板。这一发现证实了该膜在包括血浆和血小板的复杂介质中保持了其惰性血液生物材料表面。
