Olson E J, Kang J D, Fu F H, Georgescu H I, Mason G C, Evans C H
Ferguson Laboratory for Orthopaedic Research, University of Pittsburgh, Pennsylvania.
Am J Sports Med. 1988 Nov-Dec;16(6):558-70. doi: 10.1177/036354658801600602.
Growing evidence suggests that biochemical mechanisms play a role in the pathogenesis of arthritis. Cartilaginous wear particles have been shown to induce destructive enzymes and cytokines. To assess the biocompatibility of artificial ACL replacements, the effects of wear particles from the following ligaments were analyzed biochemically and histologically: GORETEX, Stryker Dacron Ligament Prosthesis, Versigraft carbon, Kennedy LAD, Xenograft, Leeds-Keio, and human patellar tendon allograft. Ligaments were frozen and ground to produce wear particles similar to those seen clinically and were added to lapine synovial cell cultures. The resulting conditioned medium was analyzed for collagenase, gelatinase, and chondrocyte activating factor (CAF) production. All of the ligaments induced significantly elevated enzyme and CAF production by the synoviocytes, with Xenograft and carbon inducing significantly higher enzyme levels than those of the other five ligaments. Five milligrams of wear particles were injected into the knees of 4 kg to 5 kg rabbits that were analyzed histologically after 14 weeks. Wear particles accumulated in the periarticular synovial folds and induced modest to severe macrophage infiltration in the synovium. A hypothetical model explaining the role of artificial ligament wear particles in the pathogenesis of arthritis is presented.
越来越多的证据表明，生化机制在关节炎的发病过程中起作用。软骨磨损颗粒已被证明可诱导破坏性酶和细胞因子。为了评估人工前交叉韧带替代物的生物相容性，对以下韧带的磨损颗粒的影响进行了生化和组织学分析：戈尔特斯（GORETEX）、史赛克涤纶韧带假体、Versigraft碳、肯尼迪LAD、异种移植物、利兹-庆应义塾韧带和人髌腱同种异体移植物。将韧带冷冻并研磨以产生与临床所见相似的磨损颗粒，并将其添加到兔滑膜细胞培养物中。分析所得的条件培养基中胶原酶、明胶酶和软骨细胞激活因子（CAF）的产生情况。所有韧带均诱导滑膜细胞产生的酶和CAF显著升高，其中异种移植物和碳诱导的酶水平明显高于其他五种韧带。将5毫克磨损颗粒注射到4至5千克兔子的膝盖中，14周后进行组织学分析。磨损颗粒积聚在关节周围的滑膜皱襞中，并在滑膜中诱导中度至重度巨噬细胞浸润。本文提出了一个解释人工韧带磨损颗粒在关节炎发病机制中作用的假说模型。
