Evans C H, Mazzocchi R A, Nelson D D, Rubash H E
Arthritis Rheum. 1984 Feb;27(2):200-7. doi: 10.1002/art.1780270212.
Human synovial fluid often contains small cartilaginous "wear particles." Previous in vitro experiments have indicated the potential involvement of these particles in the pathophysiology of arthritis. To determine whether this potential is realized under the conditions existing within joints, standard suspensions of lapine articular cartilage were injected intraarticularly into the knee joints of rabbits. Thrice-weekly injections of 1 mg allogenic cartilage produced an inflammatory arthritis, accompanied by a marked cellular effusion, in all rabbits within 5 months. The synovium became hyperplastic, discolored, and infiltrated with mononuclear inflammatory cells. Embedded particles of the injected material were seen in histologic preparations of these synovia. Organ cultures of such synovia produced 4 to 5 times more collagenase, plasminogen activator, "Pz-peptidase," neutral and acid azocaseinase, and beta-glucuronidase than did cultures of synovia from control knees injected with saline. Furthermore, the articular cartilage of knees injected with cartilaginous particles showed elevated intrinsic collagenolytic activity. Histologic examination of the articular cartilage revealed an attendant loss of metachromasy, resulting in friability, pitting, and discoloring of the cartilage. Preliminary immunoassays failed to demonstrate a systemic immune response to the injected material.
人体滑液中常含有小的软骨“磨损颗粒”。先前的体外实验表明，这些颗粒可能参与关节炎的病理生理过程。为了确定在关节内存在的条件下这种可能性是否会成为现实，将兔关节软骨的标准悬浮液关节内注射到兔膝关节中。每周三次注射1毫克同种异体软骨，在5个月内所有兔子都出现了炎症性关节炎，并伴有明显的细胞渗出。滑膜增生、变色，并被单核炎性细胞浸润。在这些滑膜的组织学标本中可见注射材料的嵌入颗粒。与注射生理盐水的对照膝关节滑膜培养物相比，这种滑膜的器官培养物产生的胶原酶、纤溶酶原激活物、“Pz肽酶”、中性和酸性偶氮酪蛋白酶以及β-葡萄糖醛酸酶多4至5倍。此外，注射软骨颗粒的膝关节软骨显示出内在胶原olytic活性升高。关节软骨的组织学检查显示伴随有异染性丧失，导致软骨易碎、出现凹坑和变色。初步免疫测定未能证明对注射材料有全身免疫反应。
