Polylactic Acid Nanofiber Scaffold Decorated with Chitosan Islandlike Topography for Bone Tissue Engineering.

In this work, a bicomponent scaffold with a core-shell and islandlike structure that combines the respective advantages of polylactic acid (PLA) and chitosan (CS) was prepared via electrospinning accompanied by automatic phase separation and crystallization. The objective of this research was to design nanosized topography with highly bioactive CS onto PLA electrospun fiber surface to improve the cell biocompatibility of the PLA fibrous membrane. The morphology, inner structure, surface composition, crystallinity, and thermodynamic analyses of nanofibers with various PLA/CS ratios were carried out, and the turning mechanism of a core-shell or islandlike topography structure was also speculated. The mineralization of hydroxyapatite and culture results of preosteoblast (MC3T3-E1) cells on the modified scaffolds indicate that the outer CS component and rough nanoscale topography on the surface of the nanofibers balanced the hydrophilicity and hydrophobicity of the fibers, enhanced their mineralization ability, and made them more beneficial for the attachment and growth of cells. Moreover, CS and "islandlike" protrusions on the fiber surface increased the alkaline phosphatase activity of the MC3T3-E1 cells seeded on the fibrous membrane and provided a more appropriate interface for cell adhesion and proliferation. These results illustrate that this kind of PLA/CS membrane has the potential in tissue engineering. More importantly, our study provides a new approach to designing PLA scaffolds, with combined topographic and bioactive modification effects at the interface between cells and materials, for biomedicine.