Laboratory of Microbiology, Department of Seafood Science, National Kaohsiung Marine University, No. 142, Hai-Chuan Rd. Nan-Tzu, Kaohsiung City 811, Taiwan, ROC.
Department of Marine Biotechnology, National Kaohsiung Marine University, No. 142, Hai-Chuan Rd. Nan-Tzu, Kaohsiung City 811, Taiwan, ROC.
Int J Syst Evol Microbiol. 2017 May;67(5):1582-1588. doi: 10.1099/ijsem.0.001768. Epub 2017 May 25.
A bacterial strain, designated Agd-32T, was isolated from a water sample taken from the Agongdian Reservoir in Taiwan and characterized using a polyphasic taxonomy approach. Cells of strain Agd-32T were Gram-stain-variable, aerobic, motile by means of peritrichous flagella, endospore-forming, rod-shaped and surrounded by a thick capsule. Strain Agd-32T contained anteiso-C15 : 0 and C16 : 0 as the predominant fatty acids. The major isoprenoid quinone was MK-7, and the polar lipid profile consisted of phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, one unidentified aminophosphoglycolipid, one unidentified glycolipid, one unidentified phospholipid and one unidentified lipid. The DNA G+C content of the genomic DNA was 56.0 mol%. Strain Agd-32T contained meso-diaminopimelic acid in the cell-wall peptidoglycan. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain Agd-32T belonged to the genus Paenibacillus and was most closely related to Paenibacillus tarimensis with sequence similarity of 96.1 % and less than 95.9 % sequence similarity with other members of the genus. On the basis of the phylogenetic inference and phenotypic data, strain Agd-32T should be classified as a representative of a novel species, for which the name Paenibacillus lacus sp. nov. is proposed. The type strain is Agd-32T (=BCRC 80902T=LMG 28836T=KCTC 33691T).
一株细菌菌株，命名为 Agd-32T，是从台湾阿公店水库的水样中分离出来的，并采用多相分类学方法进行了特征描述。菌株 Agd-32T 的细胞革兰氏染色可变，需氧，通过周生鞭毛运动，产芽孢，杆状，被厚荚膜包围。菌株 Agd-32T 含有 anteiso-C15：0 和 C16：0 作为主要脂肪酸。主要的异戊烯醌是 MK-7，极性脂类谱由磷脂酰乙醇胺、双磷脂酰甘油、磷脂酰甘油、一种未鉴定的氨基糖脂、一种未鉴定的糖脂、一种未鉴定的磷脂和一种未鉴定的脂类组成。基因组 DNA 的 G+C 含量为 56.0mol%。菌株 Agd-32T 的细胞壁肽聚糖中含有 meso-二氨基庚二酸。基于 16S rRNA 基因序列的系统发育分析表明，菌株 Agd-32T 属于类芽孢杆菌属，与类芽孢杆菌亲缘关系最近，序列相似性为 96.1%，与属内其他成员的序列相似性小于 95.9%。基于系统发育推断和表型数据，菌株 Agd-32T 应归类为一个新种的代表，因此提议将其命名为 Lacus Paenibacillus nov. sp. nov. 。模式菌株为 Agd-32T（=BCRC 80902T=LMG 28836T=KCTC 33691T）。
