Wu Yan, Dong Leyun, Weng Bingbing, Shi Xiaozhen, Wu Ningning, He Jian, Shen Zongzhuan, Jiang Jiandong, Shen Qirong
College of Life Sciences, Nanjing Agricultural University, Nanjing, Jiangsu, 210095, People's Republic of China.
Agricultural Microbial Resources Protection and Germplasm Innovation and Utilization Center of Jiangsu Province, Nanjing, Jiangsu, 210095, People's Republic of China.
Curr Microbiol. 2025 Jul 11;82(9):375. doi: 10.1007/s00284-025-04363-2.
A Gram-stain-positive, nonmotile, endospore-forming and short rod bacterium, designated as CN-4, was isolated from farm soil collected at Nanchang city, PR China. Strain CN-4 grew at 15-42 °C (optimum at 30 °C), pH 6.0-9.0 (optimum at pH 7.0) with NaCl content of 0-4% (optimum at 0.5%), and was able to fix nitrogen. The result of 16S rRNA gene sequence analysis found that strain CN-4 was most closely associated with Paenibacillus albidus Q4-3 (96.8% similarity), P. apii 7124 (96.7% similarity) and P. tianjinensis TB2019 (96.5% similarity). In the phylogenetic trees, strain CN-4 was clustered in genus Paenibacillus but formed a distinct clade. The major cellular fatty acids (≥ 5%) were anteiso-C, iso-C, C, and anteiso-C, the major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, three unidentified aminophospholipids, and five unidentified lipids. The cell wall peptidoglycan was meso-DAP, DNA G + C content was 53.7%. According to the taxonomic data, strain CN-4 represents a new species within genus Paenibacillus, as Paenibacillus nanchangensis sp. nov, with strain CN-4 (= MCCC 1K09410 = KCTC 43751) as type strain.
从中国南昌采集的农田土壤中分离出一株革兰氏染色阳性、不运动、产芽孢的短杆状细菌，命名为CN-4。菌株CN-4在15-42℃（最适温度30℃）、pH 6.0-9.0（最适pH 7.0）、NaCl含量0-4%（最适0.5%）的条件下生长，且能够固氮。16S rRNA基因序列分析结果表明，菌株CN-4与白芽孢杆菌Q4-3（相似度96.8%）、芹菜芽孢杆菌7124（相似度96.7%）和天津芽孢杆菌TB2019（相似度96.5%）关系最为密切。在系统发育树中，菌株CN-4聚类在芽孢杆菌属，但形成一个独特的分支。主要细胞脂肪酸（≥5%）为anteiso-C、iso-C、C和anteiso-C，主要极性脂为二磷脂酰甘油、磷脂酰甘油、三种未鉴定的氨基磷脂和五种未鉴定的脂类。细胞壁肽聚糖为中-DAP，DNA G+C含量为53.7%。根据分类学数据，菌株CN-4代表芽孢杆菌属内的一个新物种，命名为南昌芽孢杆菌（Paenibacillus nanchangensis sp. nov），以菌株CN-4（=MCCC 1K09410=KCTC 43751）作为模式菌株。
