Schuster W, Brennicke A
Lehrstuhl für Spezielle Botanik der Universität Tübingen, Auf der Morgenstelle 1, D-74 Tübingen, FRG.
EMBO J. 1987 Oct;6(10):2857-63. doi: 10.1002/j.1460-2075.1987.tb02587.x.
We describe an open reading frame (ORF) with high homology to reverse transcriptase in the mitochondrial genome of Oenothera. This ORF displays all the characteristics of an active plant mitochondrial gene with a possible ribosome binding site and 39% T in the third codon position. It is located between a sequence fragment from the plastid genome and one of nuclear origin downstream from the gene encoding subunit 5 of the NADH dehydrogenase. The nuclear derived sequence consists of 528 nucleotides from the small ribosomal RNA and contains an expansion segment unique to nuclear rRNAs. The plastid sequence contains part of the ribosomal protein S4 and the complete tRNA(Ser). The observation that only transcribed sequences have been found i more than one subcellular compartment in higher plants suggests that interorganellar transfer of genetic information may occur via RNA and subsequent local reverse transcription and genomic integration.
我们描述了一种在月见草线粒体基因组中与逆转录酶具有高度同源性的开放阅读框（ORF）。该ORF具有活跃植物线粒体基因的所有特征，带有一个可能的核糖体结合位点，并且第三密码子位置的胸腺嘧啶（T）含量为39%。它位于来自质体基因组的一个序列片段与核起源序列之一之间，该核起源序列位于编码NADH脱氢酶亚基5的基因下游。核衍生序列由来自小核糖体RNA的528个核苷酸组成，并包含一个核rRNA特有的扩展片段。质体序列包含核糖体蛋白S4的一部分和完整的tRNA（Ser）。在高等植物中，仅在不止一个亚细胞区室中发现转录序列的这一观察结果表明，遗传信息的细胞器间转移可能通过RNA以及随后的局部逆转录和基因组整合而发生。
