Department of Biomedical Engineering, College of Engineering, National Cheng Kung University, Taiwan; Institute of Basic Medical Science, Institute of Oral Medicine and Department of Stomatology, College of Medicine, National Cheng Kung University, Tainan, Taiwan; Medical Device Innovation Center, Taiwan Innovation Center of Medical Devices and Technology, National Cheng Kung University Hospital, National Cheng Kung University, Tainan, Taiwan.
Department of Anesthesiology, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Taiwan.
Nanomedicine. 2017 Aug;13(6):1975-1981. doi: 10.1016/j.nano.2017.05.005. Epub 2017 May 21.
Few studies have investigated the effects of iron oxide nanoparticles (NPs) on analgesia. We developed inflammatory pain models via complete Freund's adjuvant injection over the hind paw in CD1 mice. Various doses of magnetite (FeO) NPs were injected into the paw. Analgesia behavior was checked with von Frey microfilament and thermal irradiation measurements. Paw skin tissues were harvested at the maximal analgesia time point. The presence of activated white cells (CD68, myeloperoxidase) and free radical (reactive oxygen species, ROS) production was also checked. Western blotting was used to identify the changes of ROS production enzymes. FeO NPs demonstrated a dose-related analgesia effect with significant reduction in inflammatory cells, pro-inflammatory markers, and ROS production in the lesion paw. ROS production enzyme expression also declined. The results indicate that local FeO NP administration induced significant analgesia via attenuation of inflammatory cell infiltration and pro-inflammatory signaling as well as scavenging of microenvironment free radicals in a mouse inflammatory pain model.
很少有研究调查过氧化铁纳米颗粒（NPs）对镇痛的影响。我们通过在 CD1 小鼠的后爪上注射完全弗氏佐剂来建立炎症性疼痛模型。将不同剂量的磁铁矿（FeO）NPs 注入爪子。使用 von Frey 微丝和热辐射测量检查镇痛行为。在最大镇痛时间点采集爪皮组织。还检查了激活的白细胞（CD68、髓过氧化物酶）和自由基（活性氧物种，ROS）的产生情况。Western blotting 用于鉴定 ROS 产生酶的变化。FeO NPs 表现出与剂量相关的镇痛作用，显著减少了病变爪中的炎症细胞、促炎标志物和 ROS 产生。ROS 产生酶的表达也下降。结果表明，局部 FeO NP 给药通过减轻炎症细胞浸润和促炎信号以及清除小鼠炎症性疼痛模型中小环境自由基，诱导了显著的镇痛作用。
