El Ouar Ibtissem, Braicu Cornelia, Naimi Dalila, Irimie Alexendru, Berindan-Neagoe Ioana
Laboratory of Microbiological Engineering and Application, University Mentouri Constantine, Algeria.
Department of Functional Genomics and Experimental Pathology, Cancer Institute "Ion Chiricuta", Cluj-Napoca.
Pharmacogn Mag. 2017 Apr-Jun;13(50):281-285. doi: 10.4103/0973-1296.204618. Epub 2017 Apr 18.
The garden snail, , is a big land snail widely found in the Mediterranean countries. It is one of the most consumed species and widely used in zootherapy.
The present study was carried out to investigate for the first time the first time the antitumor activity of an aqueous extract from .
The effect of extract was studied on a triple negative breast cancer cell line Hs578T. Firstly, the morphological changes and the mode of cell death induced by the extract have been evaluated by microscopy and acridine orange/ethidium bromide staining. The effect of the extract at dilution 0.1% and 1% was then tested on some genes, regulators of cell death and proliferation like tumor necrosis factor α (TNFα), NF- κB, and the tumor suppressor genes P53 and PTEN.
Data demonstrate that the extract induces necrosis in tumor cells. It enhances significantly the expression of TNFα; mRNA levels were 20 and 10 times more important in treated cells compared to nontreated cells. NF-κB and PTEN were inhibited with the dilution 1% after 8 and 24 hours of treatment. P53 expression was further inhibited but only with the highest dose, after 4, 8, and 24 hours.
Our results show that extract has an antitumor activity against Hs578T cells; it is a potent stimulator for TNFα and a good inhibitor for NF-κB. AO: acridine orange; Bcl-2: B cell lymphoma 2. cDNA: complementary DNA; ELISA: enzyme linked immunosorbent assay; EB: ethidium bromide; IC50: the half maximal inhibitory concentration; mRNA: messenger RNA. MAPK: mitogen-activated protein kinase; NF-κB: nuclearfactorkappa B; PBS: phosphate buffered saline. PI3K: phospho-inositol 3 kinase; PTEN: phosphatase and tensin homolog; ROS: reactive oxygen species. RT-PCR: reverse transcription polymerase chain reaction; TNFα: tumor necrosis factor alpha. TNFR1: TNF receptor-1; TP53: tumor protein 53.
花园蜗牛，是一种大型陆地蜗牛，在地中海国家广泛分布。它是最常被食用的物种之一，并且在动物疗法中广泛应用。
本研究首次对花园蜗牛的水提取物的抗肿瘤活性进行研究。
研究了花园蜗牛提取物对三阴性乳腺癌细胞系Hs578T的作用。首先，通过显微镜检查和吖啶橙/溴化乙锭染色评估提取物诱导的形态变化和细胞死亡模式。然后测试了0.1%和1%稀释度的提取物对一些基因、细胞死亡和增殖调节因子的影响，如肿瘤坏死因子α（TNFα）、核因子κB（NF-κB）以及肿瘤抑制基因P53和PTEN。
数据表明该提取物可诱导肿瘤细胞坏死。它显著增强了TNFα的表达；与未处理细胞相比，处理后细胞中的mRNA水平高出20倍和10倍。在处理8小时和24小时后，1%稀释度的提取物抑制了NF-κB和PTEN。P53表达进一步受到抑制，但仅在最高剂量下，在4小时、8小时和24小时后出现。
我们的结果表明，花园蜗牛提取物对Hs578T细胞具有抗肿瘤活性；它是TNFα的有效刺激剂，也是NF-κB的良好抑制剂。AO：吖啶橙；Bcl-2：B细胞淋巴瘤2。cDNA：互补DNA；ELISA：酶联免疫吸附测定；EB：溴化乙锭；IC50：半数最大抑制浓度；mRNA：信使RNA。MAPK：丝裂原活化蛋白激酶；NF-κB：核因子κB；PBS：磷酸盐缓冲盐水。PI3K：磷酸肌醇3激酶；PTEN：磷酸酶和张力蛋白同源物；ROS：活性氧。RT-PCR：逆转录聚合酶链反应；TNFα：肿瘤坏死因子α。TNFR1：TNF受体-1；TP53：肿瘤蛋白53。
