Chemistry Faculty, M.V. Lomonosov Moscow State University , 119991, Moscow, Russia.
Anal Chem. 2017 Jun 20;89(12):6290-6294. doi: 10.1021/acs.analchem.7b01142. Epub 2017 May 30.
In contrast to "self-powered" (bio)sensors aiming to generate maximum energy output, we propose the systems with the lowest potential difference between the working and the counter electrodes, which in galvanic mode would provide achievement of the best analytical performance characteristics. Prussian Blue based (bio)sensors known to operate at 0.00 V versus Ag|AgCl reference, in the short-circuit regime generate the current proportional to analyte concentration. Sensitivity and dynamic range of Prussian Blue based (bio)sensors in power generation mode are, respectively, even slightly higher and wider compared to the same (bio)sensors operated in the conventional three-electrode regime powered by a potentiostat. Selectivity of the (bio)sensors in power generation mode is similarly high relative to both oxygen, allowing HO detection by its reduction, and reductants. Among the most important advantages of the proposed power generation mode is an order of magnitude decreased noise compared to performance in a conventional three-electrode setup powered by a potentiostat. Noiseless performances of Prussian Blue based (bio)sensors would open new horizons for electrochemical analysis.
与旨在产生最大能量输出的“自供电”（生物）传感器相反，我们提出了工作电极和对电极之间具有最低电位差的系统，在这种系统中，通过原电池模式可以实现最佳的分析性能特征。众所周知，基于普鲁士蓝的（生物）传感器在 0.00 V 对 Ag|AgCl 参比电极下工作，在短路状态下会产生与分析物浓度成正比的电流。与在传统的三电极系统中由恒电位仪供电运行的相同（生物）传感器相比，基于普鲁士蓝的（生物）传感器在发电模式下的灵敏度和动态范围分别更高且更宽。与氧气相比，（生物）传感器在发电模式下的选择性也很高，允许通过其还原检测 HO，并检测还原剂。与由恒电位仪供电的传统三电极设置相比，所提出的发电模式的一个重要优势是噪声降低了一个数量级。与由恒电位仪供电的传统三电极设置相比，无噪声的普鲁士蓝（生物）传感器性能将为电化学分析开辟新的视野。
