John Rani Toll, Henricson Joakim, Nilsson Gert E, Wilhelms Daniel, Anderson Chris D
Division of Neuro and Inflammation Science, Department of Clinical and Experimental Medicine, Faculty of Health Sciences, Linköping University and Department of Emergency Medicine, Local Health Care Services in Central Östergötland, Region Östergötland, 58185, Linköping, Sweden.
Division of Cell Biology, Department of Clinical and Experimental Medicine, Faculty of Health Sciences, Linköping University, Department of Dermatology and Venerology, Heart and Medicine Center, Region Östergötland.
J Biophotonics. 2018 Jan;11(1). doi: 10.1002/jbio.201700043. Epub 2017 May 24.
To use Bioengineering methodology is used to achieve, at five anatomical sites, a detailed, quantitative assessment of the return of blood content to the blanched area, during the Capillary Refill (CR) test. An observational, non-randomized, experimental study on 23 healthy subjects (14 females) was performed in our climate controlled skin physiology laboratory. Our main outcome measures were based on the chronological assessment and quantification of red blood cell concentration (RBC) after the release of blanching pressure in the CR test, using Tissue Viability Imaging (TiVi), a digital photographic technique based on polarisation spectroscopy. TiVi enabled collection of detailed data on skin RBC concentration during the CR test. The results were shown as curves with skin blood concentration (TiVi-value) on the y-axis and the time on the x-axis. Quantitative CR responses showed site and temperature variability. We also suggest possible objective endpoint values from the capillary refill curve. Detailed data on skin RBC concentration during the CR test is easily obtained and allows objective determination of end points not possible to achieve by naked eye assessment. These findings have the potential to place the utility of the CR test in a clinical setting in a new light. Picture: Regular photograph and TiVi Image showing CR test and corresponding graph for the CR response.
在毛细血管再充盈（CR）测试期间，运用生物工程方法在五个解剖部位对变白区域的血液含量恢复情况进行详细的定量评估。我们在气候可控的皮肤生理实验室对23名健康受试者（14名女性）开展了一项观察性、非随机实验研究。我们的主要观察指标基于在CR测试中释放变白压力后，使用组织活力成像（TiVi）（一种基于偏振光谱的数字摄影技术）对红细胞浓度（RBC）进行按时间顺序的评估和量化。TiVi能够在CR测试期间收集有关皮肤RBC浓度的详细数据。结果以曲线形式呈现，纵轴为皮肤血液浓度（TiVi值），横轴为时间。定量的CR反应显示出部位和温度的变异性。我们还从毛细血管再充盈曲线中提出了可能的客观终点值。在CR测试期间很容易获得有关皮肤RBC浓度的详细数据，并且能够客观确定通过肉眼评估无法实现的终点。这些发现有可能使CR测试在临床环境中的效用呈现新的面貌。图：显示CR测试的普通照片和TiVi图像以及CR反应的相应图表。
