Department of Molecular Biology and Biochemistry, University of California, Irvine, Irvine, CA, 92697-3900, USA.
Department of Chemistry, University of California, Irvine, Irvine, CA, 92697-2025, USA.
Chem Asian J. 2017 Aug 17;12(16):1985-1996. doi: 10.1002/asia.201700624. Epub 2017 Jun 28.
The reduction of CO into useful products, including hydrocarbon fuels, is an ongoing area of particular interest due to efforts to mitigate buildup of this greenhouse gas. While the industrial Fischer-Tropsch process can facilitate the hydrogenation of CO with H to form short-chain hydrocarbon products under high temperatures and pressures, a desire to perform these reactions under ambient conditions has inspired the use of biological approaches. Particularly, enzymes offer insight into how to activate and reduce CO , but only one enzyme, nitrogenase, can perform the multielectron, multiproton reduction of CO into hydrocarbons. The vanadium-containing variant, V-nitrogenase, displays especial reactivity towards the hydrogenation of CO and CO . This Focus Review discusses recent progress towards the activation and reduction of CO with three primary V-nitrogenase systems. These systems span both ATP-dependent and ATP-independent processes and utilize approaches with whole cells, isolated proteins, and extracted cofactors.
将 CO 还原为有用的产品，包括碳氢燃料，是一个特别引人关注的领域，因为人们正在努力减少这种温室气体的积累。虽然工业费托合成工艺可以促进在高温高压下用 H 将 CO 加氢形成短链碳氢化合物产品，但人们希望在环境条件下进行这些反应，这激发了对生物方法的使用。特别是，酶为如何激活和还原 CO 提供了深入的了解，但只有一种酶，氮酶，能够将 CO 多电子、多质子还原成碳氢化合物。含钒的变体 V-氮酶对 CO 和 CO 的加氢具有特别的反应活性。本重点综述讨论了在三种主要的 V-氮酶系统中激活和还原 CO 的最新进展。这些系统涵盖了依赖 ATP 和不依赖 ATP 的过程，并利用了全细胞、分离蛋白和提取辅因子的方法。
