Zheng Qinsi, Lavis Luke D
Janelia Research Campus, Howard Hughes Medical Institute, 19700 Helix Dr., Ashburn, VA 20147, USA.
Janelia Research Campus, Howard Hughes Medical Institute, 19700 Helix Dr., Ashburn, VA 20147, USA.
Curr Opin Chem Biol. 2017 Aug;39:32-38. doi: 10.1016/j.cbpa.2017.04.017. Epub 2017 May 23.
Advances in fluorescence microscopy promise to unlock details of biological systems with high spatiotemporal precision. These new techniques also place a heavy demand on the 'photon budget'-the number of photons one can extract from a sample. Improving the photostability of small molecule fluorophores using chemistry is a straightforward method for increasing the photon budget. Here, we review the (sometimes sparse) efforts to understand the mechanism of fluorophore photobleaching and recent advances to improve photostability through reducing the propensity for oxidation or through intramolecular triplet-state quenching. Our intent is to inspire a more thorough mechanistic investigation of photobleaching and the use of precise chemistry to improve fluorescent probes.
荧光显微镜技术的进步有望以高时空精度揭示生物系统的细节。这些新技术对“光子预算”（即从样品中提取的光子数量）也提出了很高的要求。利用化学方法提高小分子荧光团的光稳定性是增加光子预算的直接方法。在这里，我们回顾了（有时并不充分的）理解荧光团光漂白机制的努力，以及通过降低氧化倾向或通过分子内三重态猝灭来提高光稳定性的最新进展。我们的目的是激发对光漂白进行更深入的机理研究，并利用精确的化学方法改进荧光探针。
