Liu Qiu-Ning, Xin Zhao-Zhe, Zhu Xiao-Yu, Chai Xin-Yue, Zhao Xiao-Ming, Zhou Chun-Lin, Tang Bo-Ping
Jiangsu Key Laboratory for Bioresources of Saline Soils, Jiangsu Synthetic Innovation Center for Coastal Bio-agriculture, Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection, School of Ocean and Biological Engineering, Yancheng Teachers University, Yancheng 224007, Jiangsu Province, PR China.
Jiangsu Key Laboratory for Bioresources of Saline Soils, Jiangsu Synthetic Innovation Center for Coastal Bio-agriculture, Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection, School of Ocean and Biological Engineering, Yancheng Teachers University, Yancheng 224007, Jiangsu Province, PR China.
Biochem Biophys Res Commun. 2017 Jul 22;489(2):149-154. doi: 10.1016/j.bbrc.2017.05.115. Epub 2017 May 22.
Gene arrangements in the mitochondrial genomes (mitogenomes) of insects are conserved across the major lineages, but can be rearranged within derived groups and may provide valuable phylogenetic characters. In this study, we sequenced the entire mitogenome of Parasa consocia, a moth of the family Limacodidae (Lepidoptera: Zygaenoidea). Compared with other lepidopterans and ancestral insects, the P. consocia mitogenome features a transfer RNA gene arrangement novel among lepidopterans between the ND3 and ND5 genes: RANSEF (the underline signifies an inverted gene), which differs from the ARNSEF arrangement of ancestral insects. This rearrangement can be explained by the tandem duplication-random loss model. We inferred a phylogenetic hypothesis for the lepidopteran superfamily based on mitochondrial amino-acid sequences using the Bayesian-inference and maximum-likelihood methods. Our results showed that P. consocia belongs to the Zygaenoidea superfamily and supported the following phylogenetic relationship: Yponomeutoidea + (Tortricoidea + Zygaenoidea + (Papilionoidea + (Pyraloidea + (Noctuoidea + (Geometroidea + Bombycoidea)))))). Comparative analyses indicated that mitogenomes are a useful phylogenetic tool at the subfamily level within the order Lepidoptera. Our findings also suggest that mitogenomes are likely to represent a valuable tool for systematics in other groups of lepidopterans.
昆虫线粒体基因组（线粒体基因组）中的基因排列在主要谱系中是保守的，但在衍生类群中可能会重新排列，并可能提供有价值的系统发育特征。在本研究中，我们对刺蛾科（鳞翅目：斑蛾总科）的一种蛾类——绿刺蛾的整个线粒体基因组进行了测序。与其他鳞翅目昆虫和原始昆虫相比，绿刺蛾线粒体基因组在ND3和ND5基因之间具有一种在鳞翅目中新颖的转运RNA基因排列：RANSEF（下划线表示一个反向基因），这与原始昆虫的ARNSEF排列不同。这种重排可以用串联重复-随机丢失模型来解释。我们使用贝叶斯推断和最大似然方法，基于线粒体氨基酸序列推断了鳞翅目总科的系统发育假说。我们的结果表明，绿刺蛾属于斑蛾总科，并支持以下系统发育关系：巢蛾总科 + （卷蛾总科 + 斑蛾总科 + （凤蝶总科 + （螟蛾总科 + （夜蛾总科 + （尺蛾总科 + 蚕蛾总科）））））。比较分析表明，线粒体基因组是鳞翅目内亚科水平上一种有用的系统发育工具。我们的研究结果还表明，线粒体基因组可能是鳞翅目其他类群系统学中的一种有价值的工具。
