Jiangsu Key Laboratory for Bioresources of Saline Soils, Jiangsu Synthetic Innovation Center for Coastal Bio-agriculture, Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection, School of Ocean and Biological Engineering, Yancheng Teachers University, Yancheng, 224007, PR China; College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing, 210009, PR China.
Jiangsu Key Laboratory for Bioresources of Saline Soils, Jiangsu Synthetic Innovation Center for Coastal Bio-agriculture, Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection, School of Ocean and Biological Engineering, Yancheng Teachers University, Yancheng, 224007, PR China.
Int J Biol Macromol. 2018 Feb;107(Pt A):121-128. doi: 10.1016/j.ijbiomac.2017.08.153. Epub 2017 Sep 1.
This study was performed to better understand the phylogenetic relationships within the lepidopteran superfamily Noctuoidea. The mitochondrial genome (mitogenome) has been extensively used for studying phylogenetic relationships at different taxonomic levels. In this study, the complete mitogenome of Spilarctia subcarnea (Noctuoidea: Erebidae) was sequenced and annotated. The mitogenome is 15,441bp in length, containing 13 typical protein-coding genes (PCGs), 22 transfer RNA genes (tRNAs), two ribosomal RNA genes (rRNAs) and a noncoding control region (CR). The order and orientation of genes of S. subcarnea mitogenome with the order trnM-trnI-trnQ-nad2 is different from the ancestral insects in which trnM is located between trnQ and nad2 (trnI-trnQ-trnM-nad2). The phylogenetic relationships based on mitochondrial sequences using Bayesian inference and Maximum likelihood methods showed that S. subcarnea was closely related to Lemyra melli, supporting that S. subcarnea belongs to Erebidae. These analyses confirm that Lymantriidae should be included as subfamilies within Erebidae. The Erebidae was sister to (Nolidae+(Euteliidae+Noctuidae)); Notodontidae is sister to the other families of Noctuoidea in our study.
本研究旨在更好地了解鳞翅目夜蛾总科内的系统发育关系。线粒体基因组（mitogenome）已被广泛用于研究不同分类水平的系统发育关系。在本研究中，测定并注释了曲纹黛夜蛾（夜蛾科：天蛾科）的完整线粒体基因组。该线粒体基因组全长 15441bp，包含 13 个典型的蛋白质编码基因（PCGs）、22 个转移 RNA 基因（tRNAs）、2 个核糖体 RNA 基因（rRNAs）和一个非编码控制区（CR）。曲纹黛夜蛾线粒体基因组基因的排列和方向与 trnM 位于 trnQ 和 nad2 之间的祖先进化昆虫（trnM-trnQ-trnM-nad2）不同，其 trnM 位于 trnQ 和 nad2 之间（trnM-trnI-trnQ-nad2）。基于贝叶斯推断和最大似然法的线粒体序列构建的系统发育关系显示，曲纹黛夜蛾与美夜蛾亲缘关系较近，支持曲纹黛夜蛾属于天蛾科。这些分析证实了灯蛾科应作为天蛾科的亚科包含在内。天蛾科与（木蠹蛾科+（燕蛾科+夜蛾科））关系最近；在本研究中，Notodontidae 与夜蛾总科的其他科关系最近。
