Hu Feihu, Zhang Xiaofeng, Liu Haixia, Xu Peng, Teng Gaojun, Xiao Zhongdang
State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing, Jiangsu, China; Medical School, Southeast University, Nanjing, Jiangsu, China.
State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing, Jiangsu, China.
Biochem Biophys Res Commun. 2017 Jul 22;489(2):171-178. doi: 10.1016/j.bbrc.2017.05.119. Epub 2017 May 23.
Through a combination of biomaterials and stem cells, tissue engineering strategies for restoring and regenerating damaged peripheral nerves have recently been used to meet the challenges posed by nerve injury. In a previous study, we revealed a new way to induce neuronal differentiation of stem cells based on the temporally sequential use of miR-218 and Fibroblast Growth Factor 2 (FGF2) in vitro (FGF2-miR-218 induction approach). In the present study, we sought to investigate the application of this novel approach in repairing sciatic nerve damage in vivo. The results showed that compared with randomly oriented nanofibers, nanofibers in an aligned orientation more favored stem cell growth and elongation. Stem cells (neuronally differentiated adipose-derived mesenchymal stem cells (ASCs)) treated with the FGF2-miR-218 induction approach and integrated with 3D aligned orientation nanofibers structures as artificial nerve grafts were implanted into 10 mm transected rat sciatic nerves in vivo. The test results of immunohistochemical staining and motor function restoration indicated that the FGF2-miR-218 induction approach combined with the 3D nanofiber scaffolds facilitated the nerve regeneration. Thus, this approach could be an effective tissue engineering method for recovery of nerve damage.
通过生物材料和干细胞的结合，用于修复和再生受损周围神经的组织工程策略最近已被用于应对神经损伤带来的挑战。在先前的一项研究中，我们揭示了一种基于在体外按时间顺序使用miR-218和成纤维细胞生长因子2（FGF2）来诱导干细胞神经元分化的新方法（FGF2-miR-218诱导方法）。在本研究中，我们试图研究这种新方法在体内修复坐骨神经损伤中的应用。结果表明，与随机取向的纳米纤维相比，取向排列的纳米纤维更有利于干细胞的生长和伸长。用FGF2-miR-218诱导方法处理并与3D取向排列的纳米纤维结构整合作为人工神经移植物的干细胞（神经元分化的脂肪来源间充质干细胞（ASCs））被植入到体内10毫米横断的大鼠坐骨神经中。免疫组织化学染色和运动功能恢复的测试结果表明，FGF2-miR-218诱导方法与3D纳米纤维支架相结合促进了神经再生。因此，这种方法可能是一种有效的组织工程方法，用于恢复神经损伤。
