Fu Xiaozhe, Lin Qiang, Liang Hongru, Liu Lihui, Huang Zhibin, Li Ningqiu, Su Jianguo
College of Animal Science and Technology, Northwest A&F University, Shanxi Key Laboratory of Molecular Biology for Agriculture, Yangling, 712100, Shanxi, China.
Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of Fishery Drug Development, Ministry of Agriculture, Key Laboratory of Aquatic Animal Immune Technology, Guangzhou, 510380, Guangdong, China.
Arch Virol. 2017 Sep;162(9):2829-2834. doi: 10.1007/s00705-017-3416-z. Epub 2017 May 26.
The Rhabdoviridae is a diverse family of negative-sense single-stranded RNA viruses which infects mammals, birds, reptiles, fish, insects and plants. Herein, we reported the isolation and characterization of 6 novel viruses from diseased fish collected from China including SCRV-QY, SCRV-SS, SCRV-GM, CmRV-FS, MsRV-SS, OmbRV-JM. The typical clinical symptom of diseased fish was hemorrhaging. Efficient propagation of these isolates in a Chinese perch brain cell line was determined by means of observation of cytopathic effect, RT-PCR and electron microscopy. Sequence alignment and phylogenetic analysis of the complete G protein sequences revealed that these isolates were clustered into one monophyletic lineage belonging to the species Siniperca chuatsi rhabdovirus.
弹状病毒科是一个多样化的负链单链RNA病毒家族，可感染哺乳动物、鸟类、爬行动物、鱼类、昆虫和植物。在此，我们报告了从中国患病鱼类中分离并鉴定出6种新型病毒，包括SCRV-QY、SCRV-SS、SCRV-GM、CmRV-FS、MsRV-SS、OmbRV-JM。患病鱼类的典型临床症状是出血。通过观察细胞病变效应、RT-PCR和电子显微镜确定了这些分离株在中国鲈脑细胞系中的高效增殖。对完整G蛋白序列的序列比对和系统发育分析表明，这些分离株聚为一个单系分支，属于鳜鱼弹状病毒种。
