利用土曲霉 CICC40205 突变株对麦麸水解液进行生物转化生产衣康酸。
Production of itaconic acid by biotransformation of wheat bran hydrolysate with Aspergillus terreus CICC40205 mutant.
机构信息
School of Food Science and Engineering, Hefei University of Technology, Hefei, Anhui Province 230009, PR China; Key Laboratory for Agricultural Products Processing of Anhui Province, Hefei, Anhui Province 230009, PR China.
School of Food Science and Engineering, Hefei University of Technology, Hefei, Anhui Province 230009, PR China.
出版信息
Bioresour Technol. 2017 Oct;241:25-34. doi: 10.1016/j.biortech.2017.05.080. Epub 2017 May 17.
The replacement of the carbon source in the microbial production of itaconic acid (IA) with economic alternatives has attracted significant attention. In this study, an Aspergillus terreus CICC40205 mutant was used to increase the IA titer and decrease the citric acid titer in the wheat bran hydrolysate compared with the parental strain. The results showed that the IA titer was increased by 33.4%, whereas the citric acid titer was decreased by 75.8%, and were in accordance with those of the improved pathway of co-metabolism of glucose and xylose according to the metabolic flux analysis. Additionally, the maximum IA titer obtained in a 7-L stirred tank was 49.65gL±0.38gL. Overall, A. terreus CICC40205 showed a great potential for the industrial production of IA through the biotransformation of the wheat bran hydrolysate.
以经济替代品替代微生物生产衣康酸（IA）中的碳源引起了广泛关注。本研究利用一株米曲霉 CICC40205 突变株，与亲本菌株相比，在麦麸水解物中提高了 IA 产量，降低了柠檬酸产量。结果表明，IA 产量提高了 33.4%，柠檬酸产量降低了 75.8%，与葡萄糖和木糖共代谢途径的改进相一致，根据代谢通量分析。此外，在 7-L 搅拌釜中获得的最大 IA 产量为 49.65gL±0.38gL。总的来说，米曲霉 CICC40205 通过麦麸水解物的生物转化显示出了在 IA 工业生产中的巨大潜力。
Zotero 插件