Lee Byeong-Hoon, Chathuranga Kiramage, Uddin Md Bashir, Weeratunga Prasanna, Kim Myun Soo, Cho Won-Kyung, Kim Hong Ik, Ma Jin Yeul, Lee Jong-Soo
College of Veterinary Medicine, Chungnam National University, Daejeon, 34134, Republic of Korea.
Faculty of Veterinary and Animal Science, Sylhet Agricultural University, Sylhet, 3100, Bangladesh.
J Microbiol. 2017 Jun;55(6):488-498. doi: 10.1007/s12275-017-7088-x. Epub 2017 May 28.
Coptidis Rhizoma is derived from the dried rhizome of Ranunculaceous plants and is a commonly used traditional Chinese medicine. Although Coptidis Rhizoma is commonly used for its many therapeutic effects, antiviral activity against respiratory syncytial virus (RSV) has not been reported in detail. In this study, we evaluated the antiviral activities of Coptidis Rhizoma extract (CRE) against RSV in human respiratory tract cell line (HEp2) and BALB/c mice. An effective dose of CRE significantly reduces the replication of RSV in HEp2 cells and reduces the RSV-induced cell death. This antiviral activity against RSV was through the induction of type I interferon-related signaling and the antiviral state in HEp2 cells. More importantly, oral administration of CRE exhibited prophylactic effects in BALB/c mice against RSV. In HPLC analysis, we found the presence of several compounds in the aqueous fraction and among them; we confirmed that palmatine was related to the antiviral properties and immunemodulation effect. Taken together, an extract of Coptidis Rhizoma and its components play roles as immunomodulators and could be a potential source as promising natural antivirals that can confer protection to RSV. These outcomes should encourage further allied studies in other natural products.
黄连来源于毛茛科植物的干燥根茎，是一种常用的传统中药。尽管黄连因其多种治疗作用而被广泛使用，但其对呼吸道合胞病毒（RSV）的抗病毒活性尚未有详细报道。在本研究中，我们评估了黄连提取物（CRE）在人呼吸道细胞系（HEp2）和BALB/c小鼠中对RSV的抗病毒活性。有效剂量的CRE显著降低了RSV在HEp2细胞中的复制，并减少了RSV诱导的细胞死亡。这种对RSV的抗病毒活性是通过诱导HEp2细胞中I型干扰素相关信号和抗病毒状态实现的。更重要的是，口服CRE对BALB/c小鼠的RSV具有预防作用。在高效液相色谱分析中，我们在水相部分发现了几种化合物，其中我们证实巴马汀与抗病毒特性和免疫调节作用有关。综上所述，黄连提取物及其成分作为免疫调节剂发挥作用，可能成为有前景的天然抗病毒药物的潜在来源，可对RSV提供保护。这些结果应鼓励对其他天然产物进行进一步的相关研究。
